Inoue S, Ikehara S, Nakamura T, Good R A, Hamashima Y
J Clin Immunol. 1985 Nov;5(6):421-6. doi: 10.1007/BF00915340.
We examined the effect of heat on natural killer-cell activity and found that two different natural killer-cell subpopulations can be distinguished by their heat sensitivity; one subpopulation loses natural killer-cell activity at 41 degrees C, and the other is not affected. In a single-cell assay, the ability of natural killer cells to conjugate to K 562 cells was not affected by incubation at 41 degrees C, but the killer activity of natural killer cells after conjugating to K 562 cells was reduced at 41 degrees C. Therefore it is likely that the difference in heat sensitivity between the two subpopulations is due to postbinding cytolytic events. Tetracaine, which influences cytolytic events, was used to examine whether or not the two natural killer-cell subpopulations can be distinguished by tetracaine sensitivity. However, it was found that tetracaine inhibits natural killer-cell activity equally for both of these natural killer-cell subpopulations.
我们研究了热对自然杀伤细胞活性的影响，发现可以根据两种不同自然杀伤细胞亚群的热敏感性对其进行区分；一个亚群在41摄氏度时丧失自然杀伤细胞活性，而另一个则不受影响。在单细胞试验中，自然杀伤细胞与K562细胞结合的能力在41摄氏度孵育时不受影响，但在41摄氏度时，自然杀伤细胞与K562细胞结合后的杀伤活性降低。因此，这两个亚群热敏感性的差异可能是由于结合后溶细胞事件所致。使用影响溶细胞事件的丁卡因来检测这两个自然杀伤细胞亚群是否可以通过丁卡因敏感性进行区分。然而，发现丁卡因对这两个自然杀伤细胞亚群的自然杀伤细胞活性具有同等的抑制作用。
