Uksila J, Lassila O, Hirvonen T, Toivanen P
J Immunol. 1983 Jan;130(1):153-6.
NK cell activity of four human premature infants between 28 and 33 wk of gestation and eleven human fetuses at 9 to 22 wk of gestation was tested against the K-562 cell line in a 4-hr 51Cr-release assay. Cord blood lymphocytes from premature infants expressed well-developed NK capacity, although the level of cytotoxicity was lower than that of full-term newborns or adults. Cells prepared from fetal liver displayed cytotoxicity against K-562 targets in four out of eleven fetuses, whereas cells from fetal bone marrow, spleen, and thymus expressed only marginal or negative anti-K-562 killing. NK cell activity in the fetal liver was observed as early as at 9 wk of gestation. The functional NK capacity of premature infants and fetuses was augmented in vitro by IFN-alpha treatment. Fetal cells without spontaneous NK activity did not develop cytotoxicity against K-562 target cells in the presence of IFN-alpha. The present results corroborate the concept of the intrauterine development of human NK cell activity.
在一项4小时的51Cr释放试验中，检测了4名妊娠28至33周的人类早产儿以及11名妊娠9至22周的人类胎儿针对K-562细胞系的NK细胞活性。早产儿的脐血淋巴细胞表现出发育良好的NK能力，尽管其细胞毒性水平低于足月新生儿或成年人。从11例胎儿的肝脏中制备的细胞，有4例对K-562靶细胞显示出细胞毒性，而从胎儿骨髓、脾脏和胸腺中制备的细胞仅表现出微弱或阴性的抗K-562杀伤作用。早在妊娠9周时就观察到了胎儿肝脏中的NK细胞活性。早产儿和胎儿的功能性NK能力在体外经α干扰素处理后增强。没有自发NK活性的胎儿细胞在存在α干扰素的情况下，不会对K-562靶细胞产生细胞毒性。目前的结果证实了人类NK细胞活性在子宫内发育的概念。
