通过规避限制甲基化DNA的限制修饰系统高效构建异种基因组文库。
Efficient construction of xenogeneic genomic libraries by circumventing restriction-modification systems that restrict methylated DNA.
作者信息
Hasegawa Satoshi, Jojima Toru, Inui Masayuki
机构信息
Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, Kizugawa, Kyoto 619-0292, Japan.
Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, Kizugawa, Kyoto 619-0292, Japan; Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0101, Japan.
出版信息
J Microbiol Methods. 2018 Mar;146:13-15. doi: 10.1016/j.mimet.2018.01.005. Epub 2018 Feb 3.
An efficient method to construct xenogeneic genomic libraries with low errors and bias by circumventing restriction-modification systems that restrict methylated DNA was developed. Un-methylated genomic DNA of Escherichia coli prepared by ϕ29 DNA polymerase was introduced to Corynebacterium glutamicum R after ligation with un-methylated vector plasmids.
开发了一种有效的方法，通过规避限制甲基化DNA的限制修饰系统来构建低错误率和低偏差的异种基因组文库。用ϕ29 DNA聚合酶制备的未甲基化大肠杆菌基因组DNA与未甲基化载体质粒连接后，导入谷氨酸棒杆菌R。
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