利用源自pMEA300的穿梭载体进行分子克隆及甲醇拟无枝酸菌预苯酸脱水酶基因的特性分析。
Molecular cloning with a pMEA300-derived shuttle vector and characterization of the Amycolatopsis methanolica prephenate dehydratase gene.
作者信息
Vrijbloed J W, van Hylckama Vlieg J, van der Put N M, Hessels G I, Dijkhuizen L
机构信息
Department of Microbiology, University of Groningen, Haren, The Netherlands.
出版信息
J Bacteriol. 1995 Nov;177(22):6666-9. doi: 10.1128/jb.177.22.6666-6669.1995.
Abstract
An efficient restriction barrier for methylated DNA in the actinomycete Amycolatopsis methanolica could be avoided by using a nonmethylating Escherichia coli strain for DNA isolations. The A. methanolica prephenate dehydratase gene was cloned from a gene bank in a pMEA300-derived shuttle vector in E. coli and characterized.
摘要
通过使用非甲基化的大肠杆菌菌株进行DNA分离，可以避免在甲醇产孢囊链霉菌中形成针对甲基化DNA的有效限制屏障。从大肠杆菌中pMEA300衍生的穿梭载体中的基因文库中克隆了甲醇产孢囊链霉菌的预苯酸脱水酶基因并进行了表征。
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