Women's Health and Perinatology Research Group, Department of Clinical Medicine, Faculty of Health Sciences UiT - The Arctic University of Norway, Tromsø, Norway.
Department of Obstetrics and Gynecology, University Hospital of North Norway, Tromsø, Norway.
Acta Obstet Gynecol Scand. 2018 May;97(5):536-544. doi: 10.1111/aogs.13304. Epub 2018 Feb 15.
Laeverin is a placenta-specific protein that is normally expressed in the plasma membrane of human trophoblasts. In previous studies, we showed higher expression levels of laeverin gene in preeclamptic compared with normal placentas and found that laeverin protein was ectopically expressed in the cytoplasm of the preeclamptic placentas. Our objective was to investigate laeverin protein expression in normal and preeclamptic placentas combining immunohistochemistry and immunofluorescence.
Tissue microarray analysis of 72 placentas, obtained from 33 preeclamptic and 39 uncomplicated pregnancies, was performed. Laeverin was labeled with a specific antibody for immunohistochemistry and immunofluorescence studies.
Immunohistochemistry showed that laeverin was expressed in syncytiotrophoblasts, cytotrophoblasts and extravillous trophoblasts in all placentas examined. In preeclamptic placentas (n = 33) compared with normal placentas (n = 39), laeverin was expressed in the cell membrane in 21 (64%) vs. 21 (54%) samples (p = 0.726), in the cytoplasm in 3 (9%) vs. 2 (5%) samples (p = 0.795) and in both the cytoplasm and membrane in 9 (27%) vs. 16 (41%) samples (p = 0.0522). All placental samples that showed cytoplasmic expression of laeverin were obtained from women who delivered before 34 weeks of gestation (early-onset preeclampsia). Further, immunofluorescence studies showed laeverin expression in the cytoplasm of six preeclamptic (three early-onset and three late-onset) and one normal placenta but did not reveal any simultaneous cell membrane and cytoplasmic expression of laeverin.
Laeverin is expressed in all trophoblast cell types of normal and preeclamptic placentas. Expression pattern of laeverin in trophoblast cells is heterogeneous and not necessarily membrane-bound.
Laeverin 是一种胎盘特异性蛋白，正常表达于人类滋养层细胞的质膜上。在之前的研究中，我们发现子痫前期胎盘组织中 laeverin 基因的表达水平高于正常胎盘，并且发现 laeverin 蛋白在子痫前期胎盘的细胞质中异位表达。我们的目的是通过免疫组织化学和免疫荧光研究联合检测，来研究正常和子痫前期胎盘组织中 laeverin 蛋白的表达。
对 33 例子痫前期和 39 例非复杂妊娠获得的 72 例胎盘组织进行组织微阵列分析。使用针对 laeverin 的特异性抗体进行免疫组织化学和免疫荧光研究。
免疫组织化学显示，所有检查的胎盘组织中，laeverin 在合体滋养层细胞、细胞滋养层细胞和绒毛外滋养层细胞中均有表达。与正常胎盘（n = 39）相比，子痫前期胎盘（n = 33）中 21 例（64%）样本的 laeverin 在质膜中表达，21 例（54%）样本在质膜中表达（p = 0.726），3 例（9%）样本在细胞质中表达，2 例（5%）样本在细胞质中表达（p = 0.795），9 例（27%）样本在细胞质和质膜中表达，16 例（41%）样本在细胞质和质膜中表达（p = 0.0522）。所有显示 laeverin 细胞质表达的胎盘样本均来自于在 34 周前分娩的妇女（早发型子痫前期）。此外，免疫荧光研究显示，6 例子痫前期（3 例早发型，3 例晚发型）和 1 例正常胎盘组织中均有 laeverin 的表达，但未发现 laeverin 的质膜和细胞质同时表达。
Laeverin 在正常和子痫前期胎盘的所有滋养层细胞类型中均有表达。Laeverin 在滋养层细胞中的表达模式是异质的，不一定是膜结合的。
