Division of Life Science, Hong Kong University of Science and Technology, Hong Kong, China.
Institute for Advanced Study, Hong Kong University of Science and Technology, Hong Kong, China.
Adv Exp Med Biol. 2017;1042:189-205. doi: 10.1007/978-981-10-6955-0_9.
The eukaryotic minichromosome maintenance 2-7 complex is the core of the inactive MCM replication licensing complex and the catalytic core of the Cdc45-MCM-GINS replicative helicase. The years of effort to determine the structure of parts or the whole of the heterohexameric complex by X-ray crystallography and conventional cryo-EM produced limited success. Modern cryo-EM technology ushered in a new era of structural biology that allowed the determination of the structure of the inactive double hexamer at an unprecedented resolution of 3.8 Å. This review will focus on the fine details observed in the Mcm2-7 double hexameric complex and their implications for the function of the Mcm2-7 hexamer in its different roles during DNA replication.
真核生物的 minichromosome maintenance 2-7 复合物是无活性 MCM 复制许可复合物的核心，也是 Cdc45-MCM-GINS 复制解旋酶的催化核心。多年来，通过 X 射线晶体学和传统冷冻电镜技术来确定异六聚体复合物的部分或全部结构的努力，仅取得了有限的成功。现代冷冻电镜技术开创了结构生物学的新纪元，使无活性双六聚体复合物的结构在前所未有的分辨率 3.8Å 下得以确定。这篇综述将重点介绍在 Mcm2-7 双六聚体复合物中观察到的精细细节，以及它们对 Mcm2-7 六聚体在 DNA 复制过程中不同角色的功能的影响。
