Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Laboratorio de Bioquímica Estructural, Buenos Aires, Argentina.
CONICET- Universidad de Buenos Aires, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN). Intendente Güiraldes 2160, C1428EGA, Buenos Aires, Argentina.
Sci Rep. 2018 Jan 22;8(1):1313. doi: 10.1038/s41598-018-19495-8.
The subtle mechanisms by which protein-DNA interactions remain functional across a wide range of temperatures are largely unknown. In this work, we manually curated available information relating fully sequenced archaeal genomes with organism growth temperatures. We built a motif that represents the core promoter of each species and calculated its information content. We then studied the relation between optimal growth temperature (OGT) and information content (IC) in the promoter region.We found a positive correlation between G + C content and OGT in tRNA regions and not in overall genome. Furthermore, we found that there is a positive correlation between information content and optimal growth temperatures in Archaea. This can't be explained by an increased C+G composition nor by other obvious mechanisms. These findings suggest that increased information content could produce a positive fitness in organisms living at high temperatures. We suggest that molecular information theory may need to be adapted for hyperthermophiles.
蛋白质-DNA 相互作用在很宽的温度范围内保持功能的微妙机制在很大程度上是未知的。在这项工作中，我们手动整理了与完全测序的古细菌基因组和生物体生长温度相关的可用信息。我们构建了一个代表每个物种核心启动子的基序，并计算了它的信息含量。然后，我们研究了启动子区域中最佳生长温度 (OGT) 和信息含量 (IC) 之间的关系。我们发现 tRNA 区域中 G+C 含量与 OGT 之间存在正相关，而在整个基因组中则没有。此外，我们还发现古菌中信息含量与最佳生长温度之间存在正相关。这不能用 C+G 组成的增加或其他明显的机制来解释。这些发现表明，信息含量的增加可能会给生活在高温下的生物带来积极的适应度。我们认为，分子信息理论可能需要适应高温生物。
