人类 CPSF 复合物识别 AAUAAA 多聚腺苷酸化信号的结构基础。
Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex.
机构信息
Department of Biochemistry, University of Zurich, Zurich, Switzerland.
Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
出版信息
Nat Struct Mol Biol. 2018 Feb;25(2):135-138. doi: 10.1038/s41594-017-0020-6. Epub 2018 Jan 22.
Abstract
Mammalian mRNA biogenesis requires specific recognition of a hexanucleotide AAUAAA motif in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and polyadenylation specificity factor (CPSF) complex. Here we present a 3.1-Å-resolution cryo-EM structure of a core CPSF module bound to the PAS hexamer motif. The structure reveals the molecular interactions responsible for base-specific recognition, providing a rationale for mechanistic differences between mammalian and yeast 3' polyadenylation.
摘要
哺乳动物 mRNA 生物发生需要特定识别前体 mRNA（pre-mRNA）转录物多聚腺苷酸化信号（PAS）中的六核苷酸 AAUAAA 基序，该过程由切割和多聚腺苷酸化特异性因子（CPSF）复合物完成。在这里，我们呈现了一个结合 PAS 六聚体基序的核心 CPSF 模块的 3.1 Å 分辨率冷冻电镜结构。该结构揭示了负责碱基特异性识别的分子相互作用，为哺乳动物和酵母 3' 多聚腺苷酸化的机制差异提供了依据。
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