Department of Biochemistry, University of Zurich, Zurich, Switzerland.
Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
Nat Struct Mol Biol. 2018 Feb;25(2):135-138. doi: 10.1038/s41594-017-0020-6. Epub 2018 Jan 22.
Mammalian mRNA biogenesis requires specific recognition of a hexanucleotide AAUAAA motif in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and polyadenylation specificity factor (CPSF) complex. Here we present a 3.1-Å-resolution cryo-EM structure of a core CPSF module bound to the PAS hexamer motif. The structure reveals the molecular interactions responsible for base-specific recognition, providing a rationale for mechanistic differences between mammalian and yeast 3' polyadenylation.
哺乳动物 mRNA 生物发生需要特定识别前体 mRNA（pre-mRNA）转录物多聚腺苷酸化信号（PAS）中的六核苷酸 AAUAAA 基序，该过程由切割和多聚腺苷酸化特异性因子（CPSF）复合物完成。在这里，我们呈现了一个结合 PAS 六聚体基序的核心 CPSF 模块的 3.1 Å 分辨率冷冻电镜结构。该结构揭示了负责碱基特异性识别的分子相互作用，为哺乳动物和酵母 3' 多聚腺苷酸化的机制差异提供了依据。
