Zhang Lei, Song Weiye, Shao Di, Zhang Sui, Desai Manishi, Ness Steven, Roy Sayon, Yi Ji
Department of Medicine, Boston University School of Medicine, Boston MA, 02118, USA.
Danna-Farber Cancer Institute, Boston MA, 02215, USA.
Biomed Opt Express. 2017 Dec 4;9(1):25-40. doi: 10.1364/BOE.9.000025. eCollection 2018 Jan 1.
While fluorescent contrast is widely used in ophthalmology, three-dimensional (3D) fluorescence retinal imaging over a large field of view (FOV) has been challenging. In this paper, we describe a novel oblique scanning laser ophthalmoscopy (oSLO) technique that provides 3D volumetric fluorescence retinal imaging with only one raster scan. The technique utilizes scanned oblique illumination and angled detection to obtain fluorescent cross-sectional images, analogous to optical coherence tomography (OCT) line scans (or B-scans). By breaking the coaxial optical alignment used in conventional retinal imaging modalities, depth resolution is drastically improved. To demonstrate the capability of oSLO, we have performed volumetric fluorescein angiography (FA) of the rat retina with ~25μm depth resolution and over a 30° FOV. Using depth segmentation, oSLO can obtain high contrast images of the microvasculature down to single capillaries in 3D. The multi-modal nature of oSLO also allows for seamless combination with simultaneous OCT angiography.
虽然荧光造影在眼科中被广泛使用，但在大视野（FOV）下进行三维（3D）荧光视网膜成像一直具有挑战性。在本文中，我们描述了一种新型的斜扫描激光检眼镜（oSLO）技术，该技术仅通过一次光栅扫描就能提供3D视网膜体积荧光成像。该技术利用扫描的斜向照明和倾斜检测来获取荧光横截面图像，类似于光学相干断层扫描（OCT）线扫描（或B扫描）。通过打破传统视网膜成像模式中使用的同轴光学对准，深度分辨率得到了显著提高。为了证明oSLO的能力，我们对大鼠视网膜进行了体积荧光血管造影（FA），深度分辨率约为25μm，视野为30°。通过深度分割，oSLO可以在3D中获得低至单个毛细血管的高对比度微血管图像。oSLO的多模态特性还允许与同步OCT血管造影无缝结合。
