使用三次谐波产生显微镜对动脉粥样硬化斑块进行无标记成像。
Label-free imaging of atherosclerotic plaques using third-harmonic generation microscopy.
作者信息
Small David M, Jones Jason S, Tendler Irwin I, Miller Paul E, Ghetti Andre, Nishimura Nozomi
机构信息
Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, 526 N. Campus Rd., Ithaca, NY 14853, USA.
Anabios Corporation, 3030 Bunker Hill St., San Diego, CA 92109, USA.
出版信息
Biomed Opt Express. 2017 Dec 13;9(1):214-229. doi: 10.1364/BOE.9.000214. eCollection 2018 Jan 1.
Abstract
Multiphoton microscopy using laser sources in the mid-infrared range (MIR, 1,300 nm and 1,700 nm) was used to image atherosclerotic plaques from murine and human samples. Third harmonic generation (THG) from atherosclerotic plaques revealed morphological details of cellular and extracellular lipid deposits. Simultaneous nonlinear optical signals from the same laser source, including second harmonic generation and endogenous fluorescence, resulted in label-free images of various layers within the diseased vessel wall. The THG signal adds an endogenous contrast mechanism with a practical degree of specificity for atherosclerotic plaques that complements current nonlinear optical methods for the investigation of cardiovascular disease. Our use of whole-mount tissue and backward scattered epi-detection suggests THG could potentially be used in the future as a clinical tool.
摘要
使用中红外波段（MIR，1300纳米和1700纳米）激光源的多光子显微镜用于对小鼠和人类样本中的动脉粥样硬化斑块进行成像。动脉粥样硬化斑块的三次谐波产生（THG）揭示了细胞和细胞外脂质沉积物的形态细节。来自同一激光源的同时非线性光学信号，包括二次谐波产生和内源性荧光，产生了病变血管壁内各层的无标记图像。THG信号增加了一种内源性对比机制，对动脉粥样硬化斑块具有实际程度的特异性，补充了当前用于研究心血管疾病的非线性光学方法。我们对整装组织和背向散射落射检测的使用表明，THG未来有可能用作临床工具。
相似文献
1
Label-free imaging of atherosclerotic plaques using third-harmonic generation microscopy.使用三次谐波产生显微镜对动脉粥样硬化斑块进行无标记成像。
Biomed Opt Express. 2017 Dec 13;9(1):214-229. doi: 10.1364/BOE.9.000214. eCollection 2018 Jan 1.
2
In vivo optical biopsy of hamster oral cavity with epi-third-harmonic-generation microscopy.利用落射式三次谐波产生显微镜对仓鼠口腔进行体内光学活检。
Opt Express. 2006 Jun 26;14(13):6178-87. doi: 10.1364/oe.14.006178.
3
Visualization of mitochondria in cardiomyocytes by simultaneous harmonic generation and fluorescence microscopy.通过同步谐波产生和荧光显微镜观察心肌细胞中的线粒体。
Opt Express. 2005 Oct 3;13(20):8263-76. doi: 10.1364/opex.13.008263.
4
Label-free molecular imaging of atherosclerotic lesions using multimodal nonlinear optical microscopy.使用多模态非线性光学显微镜对动脉粥样硬化病变进行无标记分子成像。
J Biomed Opt. 2007 Sep-Oct;12(5):054007. doi: 10.1117/1.2795437.
5
Third harmonic generation microscopy of cells and tissue organization.细胞与组织结构的三次谐波产生显微镜检查
J Cell Sci. 2016 Jan 15;129(2):245-55. doi: 10.1242/jcs.152272. Epub 2016 Jan 7.
6
Multiphoton microscopy for label-free multicolor imaging of peripheral nerve.多光子显微镜用于周围神经的无标记多色成像。
J Biomed Opt. 2022 May;27(5). doi: 10.1117/1.JBO.27.5.056501.
7
Label-free 3D visualization of cellular and tissue structures in intact muscle with second and third harmonic generation microscopy.无标记 3D 可视化完整肌肉中的细胞和组织结构，采用二次和三次谐波产生显微镜。
PLoS One. 2011;6(11):e28237. doi: 10.1371/journal.pone.0028237. Epub 2011 Nov 28.
8
Imaging lipid bodies in cells and tissues using third-harmonic generation microscopy.使用三次谐波产生显微镜对细胞和组织中的脂滴进行成像。
Nat Methods. 2006 Jan;3(1):47-53. doi: 10.1038/nmeth813.
9
Harmonic optical microscopy and fluorescence lifetime imaging platform for multimodal imaging.用于多模态成像的谐波光学显微镜和荧光寿命成像平台。
Microsc Res Tech. 2012 Oct;75(10):1383-94. doi: 10.1002/jemt.22078. Epub 2012 May 31.
10
Spectroscopic second and third harmonic generation microscopy using a femtosecond laser source in the third near-infrared (NIR-III) optical window.在第三近红外（NIR-III）光学窗口中使用飞秒激光源的光谱二次和三次谐波产生显微镜。
Biomed Opt Express. 2022 Jan 11;13(2):694-708. doi: 10.1364/BOE.446273. eCollection 2022 Feb 1.
引用本文的文献
1
HIV-1 transmission: modelling and direct visualization in the third dimension.HIV-1 传播：三维建模和直接可视化。
Microscopy (Oxf). 2023 Jun 8;72(3):164-177. doi: 10.1093/jmicro/dfad014.
2
Label-free third harmonic generation imaging and quantification of lipid droplets in live filamentous fungi.无标记的三次谐波产生成像和活丝状真菌中脂滴的定量分析。
Sci Rep. 2022 Nov 5;12(1):18760. doi: 10.1038/s41598-022-23502-4.
3
Multiphoton microscopy providing pathological-level quantification of myocardial fibrosis in transplanted human heart.多光子显微镜对移植人心肌纤维化进行病理水平的定量分析。
Lasers Med Sci. 2022 Sep;37(7):2889-2898. doi: 10.1007/s10103-022-03557-5. Epub 2022 Apr 8.
4
Label-Free Multiphoton Microscopy: Much More Than Fancy Images.无标记多光子显微镜：不仅仅是花哨的图像。
Int J Mol Sci. 2021 Mar 6;22(5):2657. doi: 10.3390/ijms22052657.
5
Photoluminescence of InAs/GaAs quantum dots under direct two-photon excitation.直接双光子激发下InAs/GaAs量子点的光致发光
Sci Rep. 2020 Jul 2;10(1):10930. doi: 10.1038/s41598-020-67961-z.
6
Label-free assessment of hemodynamics in individual cortical brain vessels using third harmonic generation microscopy.使用三次谐波产生显微镜对个体皮质脑血管中的血流动力学进行无标记评估。
Biomed Opt Express. 2020 Apr 23;11(5):2665-2678. doi: 10.1364/BOE.385848. eCollection 2020 May 1.
7
Miniaturized multimodal multiphoton microscope for simultaneous two-photon and three-photon imaging with a dual-wavelength Er-doped fiber laser.用于双波长掺铒光纤激光器同时进行双光子和三光子成像的小型化多模态多光子显微镜。
Biomed Opt Express. 2020 Jan 6;11(2):624-635. doi: 10.1364/BOE.381473. eCollection 2020 Feb 1.
8
An intravital window to image the colon in real time.实时成像结肠的活体窗口。
Nat Commun. 2019 Dec 11;10(1):5647. doi: 10.1038/s41467-019-13699-w.
9
Harmonic Generation Microscopy 2.0: New Tricks Empowering Intravital Imaging for Neuroscience.谐波产生显微镜2.0：助力神经科学活体成像的新方法
Front Mol Biosci. 2019 Oct 9;6:99. doi: 10.3389/fmolb.2019.00099. eCollection 2019.
10
Second and third harmonic generation microscopy visualizes key structural components in fresh unprocessed healthy human breast tissue.二次和三次谐波产生显微镜可观察到新鲜未经处理的健康人乳腺组织中的关键结构成分。
J Biophotonics. 2019 Jun;12(6):e201800297. doi: 10.1002/jbio.201800297. Epub 2019 Mar 21.
本文引用的文献
1
Assessment of atherosclerotic luminal narrowing of coronary arteries based on morphometrically generated visual guides.基于形态学生成的可视导丝评估冠状动脉粥样硬化性管腔狭窄
Cardiovasc Pathol. 2017 Jul-Aug;29:53-60. doi: 10.1016/j.carpath.2017.05.005. Epub 2017 May 30.
2
Adaptive optical fluorescence microscopy.自适应光学荧光显微镜。
Nat Methods. 2017 Mar 31;14(4):374-380. doi: 10.1038/nmeth.4218.
3
In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain.在完整小鼠大脑深处用 GCaMP6 标记的神经元的体内三光子成像。
Nat Methods. 2017 Apr;14(4):388-390. doi: 10.1038/nmeth.4183. Epub 2017 Feb 20.
4
Multimodal optoacoustic and multiphoton microscopy of human carotid atheroma.人类颈动脉粥样硬化的多模态光声和多光子显微镜检查
Photoacoustics. 2016 Aug 4;4(3):102-111. doi: 10.1016/j.pacs.2016.07.001. eCollection 2016 Sep.
5
Calcific Aortic Valve Disease Is Associated with Layer-Specific Alterations in Collagen Architecture.钙化性主动脉瓣疾病与胶原蛋白结构的层特异性改变有关。
PLoS One. 2016 Sep 29;11(9):e0163858. doi: 10.1371/journal.pone.0163858. eCollection 2016.
6
Intravital assessment of myelin molecular order with polarimetric multiphoton microscopy.利用偏振多光子显微镜对髓鞘分子有序性进行活体评估。
Sci Rep. 2016 Aug 19;6:31685. doi: 10.1038/srep31685.
7
Characterization of Proliferating Lesion-Resident Cells During All Stages of Atherosclerotic Growth.动脉粥样硬化发展各阶段增殖性病变驻留细胞的特征分析
J Am Heart Assoc. 2016 Aug 15;5(8):e003945. doi: 10.1161/JAHA.116.003945.
8
Comparing Raman and fluorescence lifetime spectroscopy from human atherosclerotic lesions using a bimodal probe.使用双峰探针比较人动脉粥样硬化病变的拉曼光谱和荧光寿命光谱。
J Biophotonics. 2016 Sep;9(9):958-66. doi: 10.1002/jbio.201500341. Epub 2016 Mar 22.
9
Clinical Characterization of Coronary Atherosclerosis With Dual-Modality OCT and Near-Infrared Autofluorescence Imaging.冠状动脉粥样硬化的双模态光学相干断层扫描和近红外自发荧光成像的临床特征
JACC Cardiovasc Imaging. 2016 Nov;9(11):1304-1314. doi: 10.1016/j.jcmg.2015.11.020. Epub 2016 Mar 9.
10
Adaptive optics in multiphoton microscopy: comparison of two, three and four photon fluorescence.多光子显微镜中的自适应光学：双光子、三光子和四光子荧光的比较
Opt Express. 2015 Nov 30;23(24):31472-83. doi: 10.1364/OE.23.031472.
Zotero 插件