Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Nat Chem. 2018 Feb;10(2):212-217. doi: 10.1038/nchem.2878. Epub 2017 Nov 6.
Prebiotic phosphorylation of (pre)biological substrates under aqueous conditions is a critical step in the origins of life. Previous investigations have had limited success and/or require unique environments that are incompatible with subsequent generation of the corresponding oligomers or higher-order structures. Here, we demonstrate that diamidophosphate (DAP)-a plausible prebiotic agent produced from trimetaphosphate-efficiently (amido)phosphorylates a wide variety of (pre)biological building blocks (nucleosides/tides, amino acids and lipid precursors) under aqueous (solution/paste) conditions, without the need for a condensing agent. Significantly, higher-order structures (oligonucleotides, peptides and liposomes) are formed under the same phosphorylation reaction conditions. This plausible prebiotic phosphorylation process under similar reaction conditions could enable the systems chemistry of the three classes of (pre)biologically relevant molecules and their oligomers, in a single-pot aqueous environment.
在水相条件下，（前）生物底物的前生物磷酸化是生命起源的关键步骤。以前的研究虽然取得了一定的成功，但/或需要独特的环境，这些环境与随后生成相应的低聚物或更高阶结构不兼容。在这里，我们证明二氨基磷酸酯（DAP）-一种可能的前生物试剂，可从三磷酸酯中高效地（酰胺）磷酸化各种（前）生物构建块（核苷/盐、氨基酸和脂质前体），无需缩合剂。重要的是，在相同的磷酸化反应条件下形成了更高阶的结构（寡核苷酸、肽和脂质体）。在类似的反应条件下，这种合理的前生物磷酸化过程可以使三类（前）生物相关分子及其低聚物的系统化学在单一的水相环境中进行。
