Sahin Kazim, Orhan Cemal, Tuzcu Mehmet, Hayirli Armagan, Komorowski James R, Sahin Nurhan
Department of Animal Nutrition and Nutritional Disorders, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey.
Division of Biology, Faculty of Science, Firat University, Elazig, Turkey.
PLoS One. 2018 Jan 23;13(1):e0189329. doi: 10.1371/journal.pone.0189329. eCollection 2018.
The effects of supplementation of arginine-silicate-inositol complex (ASI; 49.5-8.2-25 g/kg, respectively) to laying hens were investigated with respect to eggshell quality, calcium (Ca) balance, and expression of duodenal proteins related to Ca metabolism (calbindin and tight junction proteins). A total of 360 laying hens, 25 weeks old, were divided into 3 groups consisting of 6 replicate of cages, 20 birds per cage. The groups were fed a basal diet and the basal diet supplemented with 500 or 1000 mg ASI complex per kilogram for 90 days. Data were analyzed by ANCOVA using data during the first week of the adaptation period as covariates. As the ASI complex supplementation level increased, there were increases in feed intake (P < 0.0001), egg production (P < 0.001), egg weight (P < 0.0001) and eggshell weight (P < 0.001) weight, and shell thickness (P < 0.001) and decreases in feed conversion ratio and cracked egg percentage (P < 0.0001 for both). Concentrations of serum osteocalcin (P < 0.0001), vitamin D (P < 0.0001), calcium (P < 0.001), phosphorus (P < 0.001), and alkaline phosphatase (P < 0.008) as well as amounts of calcium retention (P < 0.0001) and eggshell calcium deposition (P < 0.001), and Ca balance (P < 0.0001) increased, whereas amount of calcium excretion (P < 0.001) decreased linearly in a dose-dependent manner. The ASI complex supplementation increased expressions of calcium transporters (calbindin-D28k, N sodium-calcium exchanger, plasma membrane calcium ATPase, and vitamin D receptor) and tight junction proteins (zonula occludens-1 and occludin) in the duodenum in a linear fashion (P < 0.0001 for all). In conclusion, provision of dietary ASI complex to laying hens during the peak laying period improved eggshell quality through improving calcium utilization as reflected by upregulation of genes related to the calcium metabolism. Further studies are needed to elucidate the contribution of each of the ASI complex ingredients.
研究了在蛋鸡日粮中添加精氨酸 - 硅酸盐 - 肌醇复合物（ASI；分别为49.5 - 8.2 - 25 g/kg）对蛋壳质量、钙（Ca）平衡以及十二指肠中与钙代谢相关蛋白质（钙结合蛋白和紧密连接蛋白）表达的影响。选取360只25周龄的蛋鸡，分为3组，每组6个重复笼，每个笼20只鸡。这些组分别饲喂基础日粮以及在基础日粮中每千克添加500或1000 mg ASI复合物，持续90天。使用适应期第一周的数据作为协变量，通过协方差分析对数据进行分析。随着ASI复合物添加水平的增加，采食量（P < 0.0001）、产蛋量（P < 0.001）、蛋重（P < 0.0001）和蛋壳重（P < 0.001）、蛋壳厚度（P < 0.001）均增加，饲料转化率和破蛋率降低（两者P < 0.0001）。血清骨钙素（P < 0.0001）、维生素D（P < 0.0001）、钙（P < 0.001）、磷（P < 0.001）和碱性磷酸酶（P < 0.008）的浓度以及钙保留量（P < 0.0001）、蛋壳钙沉积量（P < 0.001）和钙平衡（P < 0.0001）均增加，而钙排泄量（P < 0.001）呈剂量依赖性线性下降。添加ASI复合物使十二指肠中钙转运蛋白（钙结合蛋白 - D28k、钠钙交换体、质膜钙ATP酶和维生素D受体）和紧密连接蛋白（闭合蛋白 - 1和闭合蛋白）的表达呈线性增加（所有P < 0.0001）。总之，在产蛋高峰期给蛋鸡提供日粮ASI复合物可通过提高与钙代谢相关基因的表达所反映的钙利用率来改善蛋壳质量。需要进一步研究以阐明ASI复合物各成分的作用。
