Okuno Yoshiharu, Marumoto Shinsuke, Tsurumi Jun, Miyazawa Mitsuo
a Department of Applied Chemistry and Biochemistry , National Institute of Technology, Wakayama Collage , Gobo-shi, Japan.
b Joint Research Centre , Kindai University (Former name :Kinki University) , Higashiosaka-shi, Japan.
Nat Prod Res. 2019 May;33(10):1518-1521. doi: 10.1080/14786419.2017.1422180. Epub 2018 Jan 24.
The biotransformation of (+)-isofraxinellone () by was investigated. Compound was transformed to only one new compound The structure of 2 was identified as (-)-(4)-4-hydroxyisofraxinellone which was regio- and stereo-selective hydroxylated at the C-4 position by IR, EI-MS 1D and 2D NMR. Absolute configuration of hydroxyl group at the C-4 position was detected by modified Mosher's method. Antifeedant activity of compounds and against larvae of was assayed. These compounds showed potent antifeedant activity and ED (50% of effective dose) values were 3.91 and 4.43 μg/cm, respectively.
研究了（+）-异白鲜碱（）的生物转化。化合物被转化为仅一种新化合物，通过红外光谱、电子轰击质谱、一维和二维核磁共振确定化合物2的结构为（-）-（4）-4-羟基异白鲜碱，其在C-4位进行了区域和立体选择性羟基化。通过改进的莫舍尔方法检测C-4位羟基的绝对构型。测定了化合物和对幼虫的拒食活性。这些化合物表现出较强的拒食活性，半数有效剂量（ED）值分别为3.91和4.43μg/cm。
