果糖2,6 - 二磷酸在进食、饥饿及再喂食的泌乳大鼠乳腺中的作用
Role of fructose 2,6-bisphosphate in mammary gland of fed, starved and re-fed lactating rats.
作者信息
Ward S, Kuhn N J
出版信息
Biochem J. 1985 Dec 15;232(3):931-4. doi: 10.1042/bj2320931.
Abstract
The fructose 2,6-bisphosphate (Fru-2,6-P2) content and intracellular concentration of lactating mammary gland was measured in fed, starved and re-fed rats. There was little or no change on starvation, and about 1.5-fold rise on re-feeding, contrasting with estimated glycolytic changes of about 10-fold. The 6-phosphofructokinase (PFK-1) activity of mammary extracts was highly sensitive to added Fru-2,6-P2 under all conditions examined, and appeared to approach saturation at physiological concentrations of this effector. The activity of mammary PFK-1 measured under optimal and 'physiological' conditions suggested that this enzyme operates in vivo at about 24% of maximal rate, and is likely to be an important rate-limiting factor in mammary glycolysis.
摘要
在喂食、饥饿和再喂食的大鼠中测量了泌乳乳腺的果糖2,6 -二磷酸（Fru - 2,6 - P2）含量和细胞内浓度。饥饿时变化很小或没有变化，再喂食时升高约1.5倍，这与估计的糖酵解变化约10倍形成对比。在所研究的所有条件下，乳腺提取物的6 -磷酸果糖激酶（PFK - 1）活性对添加的Fru - 2,6 - P2高度敏感，并且在该效应物的生理浓度下似乎接近饱和。在最佳和“生理”条件下测量的乳腺PFK - 1活性表明，该酶在体内以最大速率的约24%起作用，并且可能是乳腺糖酵解中的一个重要限速因子。
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