College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Institute of Biotechnology, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Int J Mol Sci. 2018 Jan 24;19(2):350. doi: 10.3390/ijms19020350.
Banana wilt caused by f. sp. () is one of the most destructive soil-borne diseases. In this study, young tissue-cultured plantlets of banana ( spp. AAA) cultivars differing in susceptibility were used to reveal their differential responses to this pathogen using digital gene expression (DGE). Data were evaluated by various bioinformatic tools (Venn diagrams, gene ontology (GO) annotation and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses) and immunofluorescence labelling method to support the identification of gene candidates determining the resistance of banana against . Interestingly, we have identified as an important gene involved in both constitutive and induced resistance. We also identified new genes involved in the resistance of banana to , including several other transcription factors (TFs), pathogenesis-related () genes and some genes related to the plant cell wall biosynthesis or degradation (e.g., pectinesterases, β-glucosidases, xyloglucan endotransglucosylase/hydrolase and endoglucanase). The resistant banana cultivar shows activation of and genes as well as formation of different constitutive cell barriers to restrict spreading of the pathogen. These data suggest new mechanisms of banana resistance to .
由（）引起的香蕉枯萎病是最具破坏性的土传病害之一。在这项研究中，使用对该病原体易感性不同的不同品种（AAA 组）的组织培养幼苗，利用数字基因表达（DGE）揭示它们对该病原体的差异反应。通过各种生物信息学工具（Venn 图、基因本体论（GO）注释和京都基因与基因组百科全书（KEGG）途径分析）和免疫荧光标记方法对数据进行评估，以支持鉴定决定香蕉对（）抗性的基因候选者。有趣的是，我们已经鉴定出（）作为涉及组成型和诱导抗性的重要基因。我们还鉴定了与香蕉对（）抗性相关的新基因，包括几个其他转录因子（TFs）、与发病机制相关（）的基因和一些与植物细胞壁生物合成或降解相关的基因（例如果胶酯酶、β-葡萄糖苷酶、木葡聚糖内切糖基转移酶/水解酶和内切葡聚糖酶）。抗性香蕉品种表现出（）和（）基因的激活以及形成不同的组成型细胞屏障，以限制病原体的扩散。这些数据表明了香蕉对（）抗性的新机制。
