Zhu Rui, Yang Xueqin, Zhang Jianjun, Xu Danwen, Fan Jiawen, Shi Huoying, Wang Shifeng, Liu Xiufan
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
J Vet Sci. 2018 May 31;19(3):406-415. doi: 10.4142/jvs.2018.19.3.406.
The subtype H9N2 avian influenza virus greatly threatens the Chinese poultry industry, even with annual vaccination. Waterfowl can be asymptomatically infected with the H9N2 virus. In this study, three H9N2 virus strains, designated A/Goose/Jiangsu/YZ527/2011 (H9N2, Gs/JS/YZ527/11), A/Goose/Jiangsu/SQ119/2012 (H9N2, Gs/JS/SQ119/12), and A/Goose/Jiangsu/JD564/2012 (H9N2, Gs/JS/JD564/12), were isolated from domestic geese. Molecular characterization of the three isolates showed that the Gs/JS/YZ527/11 virus is a double-reassortant virus, combining genes of A/Quail/Hong Kong/G1/97 (H9N2, G1/97)-like and A/Chicken/Shanghai/F/98 (H9N2, F/98)-like; the Gs/JS/SQ119/12 virus is a triple-reassortant virus combining genes of G1/97-like, F/98-like, and A/Duck/Shantou/163/2004 (H9N2, ST/163/04)-like. The sequences of Gs/JS/JD564/12 share high homology with those of the F/98 virus, except for the neuraminidase gene, whereas the internal genes of Gs/JS/YZ527/11 and Gs/JS/SQ119/12 are closely related to those of the H7N9 viruses. An infectivity analysis of the three isolates showed that Gs/JS/SQ119/12 and Gs/JS/YZ527/11 replicated well, with seroconversion, in geese and chickens, the Gs/JS/JD564/12 did not infect well in geese or chickens, and the F/98 virus only infected chickens, with seroconversion. Emergence of these new reassortant H9N2 avian influenza viruses indicates that these viruses can infect both chicken and goose and can produce different types of lesions in each species.
H9N2亚型禽流感病毒对中国家禽业构成了巨大威胁，即便每年都进行疫苗接种。水禽可无症状感染H9N2病毒。在本研究中，从家鹅中分离出三株H9N2病毒株，分别命名为A/Goose/Jiangsu/YZ527/2011（H9N2，Gs/JS/YZ527/11）、A/Goose/Jiangsu/SQ119/2012（H9N2，Gs/JS/SQ119/12）和A/Goose/Jiangsu/JD564/2012（H9N2，Gs/JS/JD564/12）。对这三株分离株的分子特征分析表明，Gs/JS/YZ527/11病毒是一种双重重配病毒，结合了A/Quail/Hong Kong/G1/97（H9N2，G1/97）样和A/Chicken/Shanghai/F/98（H9N2，F/98）样的基因；Gs/JS/SQ119/12病毒是一种三重重配病毒，结合了G1/97样、F/98样和A/Duck/Shantou/163/2004（H9N2，ST/163/04）样的基因。Gs/JS/JD564/12的序列与F/98病毒的序列具有高度同源性，除了神经氨酸酶基因，而Gs/JS/YZ527/11和Gs/JS/SQ119/12的内部基因与H7N9病毒的内部基因密切相关。对这三株分离株的感染性分析表明，Gs/JS/SQ119/12和Gs/JS/YZ527/11在鹅和鸡中复制良好，并出现了血清转化，Gs/JS/JD564/12在鹅或鸡中感染性不佳，而F/98病毒仅感染鸡并出现血清转化。这些新的重配H9N2禽流感病毒的出现表明，这些病毒既能感染鸡也能感染鹅，并能在每个物种中产生不同类型的病变。
