IBB-Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Phytother Res. 2012 Sep;26(9):1366-70. doi: 10.1002/ptr.4591. Epub 2012 Feb 1.
Moringa oleifera is an important source of antioxidants, tools in nutritional biochemistry that could be beneficial for human health; the leaves and flowers are used by the population with great nutritional importance. This work investigates the antioxidant activity of M. oleifera ethanolic (E1) and saline (E2) extracts from flowers (a), inflorescence rachis (b), seeds (c), leaf tissue (d), leaf rachis (e) and fundamental tissues of stem (f). The radical scavenging capacity (RSC) of extracts was determined using dot-blots on thin layer chromatography stained with a 0.4 mM 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) solution; spectrophotometric assays were recorded (515 nm). Antioxidant components were detected in all E1 and E2 from a, b and d. The best RSC was obtained with E1d; the antioxidants present in E2 reacted very slowly with DPPH. The chromatogram revealed by diphenylborinate-2-ethylamine methanolic solution showed that the ethanolic extract from the flowers, inflorescence rachis, fundamental tissue of stem and leaf tissue contained at least three flavonoids; the saline extract from the flowers and leaf tissue revealed at least two flavonoids. In conclusion, M. oleifera ethanolic and saline extracts contain antioxidants that support the use of the plant tissues as food sources.
辣木是抗氧化剂的重要来源，抗氧化剂是营养生物化学中的工具，可能对人类健康有益；叶子和花被人们大量用作具有重要营养价值的食物。本研究调查了辣木叶（a）、花序轴（b）、种子（c）、叶组织（d）、叶轴（e）和茎基本组织（f）的辣木乙醇（E1）和盐水（E2）提取物的抗氧化活性。采用薄层层析斑点法，用 0.4 mM 1,1-二苯基-2-苦基肼自由基（DPPH）溶液染色，测定提取物的自由基清除能力（RSC）；记录分光光度测定（515nm）。在所有 E1 和 E2 中均检测到 a、b 和 d 中的抗氧化成分。E1d 的 RSC 最佳；E2 中的抗氧化剂与 DPPH 反应非常缓慢。二苯并硼酸盐-2-乙胺甲醇溶液显示的色谱图表明，花、花序轴、茎的基本组织和叶组织的乙醇提取物至少含有三种类黄酮；花和叶组织的盐水提取物至少含有两种类黄酮。综上所述，辣木的乙醇和盐水提取物含有抗氧化剂，支持将植物组织作为食物来源。
