Pornputtapitak Warangkana, Pantakitcharoenkul Jaturavit, Panpakdee Ratchada, Teeranachaideekul Veerawat, Sinchaipanid Nuttanan
Department of Chemical Engineering, Faculty of Engineering, Mahidol University.
Department of Pharmacy, Faculty of Pharmacy, Mahidol University.
J Oleo Sci. 2018 Feb 1;67(2):125-133. doi: 10.5650/jos.ess17113. Epub 2018 Jan 23.
Leum Pua is native Thai glutinous rice that contains antioxidants higher than white rice and other colored rice. One of the major antioxidants in rice brans is γ-oryzanol (GO). In this study, Leum Pua glutinous rice bran was extracted by different solvents. Oleic acid (40 g/100 g extract), linoleic acid (30 g/100 g extract), and palmitic acid (~20 g/100 g extract) were found to be major lipid components in the extracts. Methanol extract showed less variety of lipid components compared to the others. However, hexane extract showed the highest percent of γ-oryzanol compared to other solvents. Therefore, the hexane extract was selected to prepare nanostructured lipid carriers (NLC). The prepared NLC had small particles in the size range of 142.9 ± 0.4 nm for extract-loaded NLC and 137.1 ± 0.5 nm for GO-loaded NLC with narrow size distribution (PI < 0.1) in both formulations. The release profile of extract-loaded NLC formulation was slightly higher than GO-loaded NLC formulation. However, they did not follow the Higuchi model because of small amounts of γ-oryzanol loaded in NLC particles.
Leum Pua是泰国本土的糯米，其抗氧化剂含量高于白米和其他有色米。米糠中的主要抗氧化剂之一是γ-谷维素（GO）。在本研究中，用不同溶剂提取Leum Pua糯米糠。提取物中的主要脂质成分是油酸（约40克/100克提取物）、亚油酸（约30克/100克提取物）和棕榈酸（约20克/100克提取物）。与其他提取物相比，甲醇提取物的脂质成分种类较少。然而，与其他溶剂相比，己烷提取物的γ-谷维素含量最高。因此，选择己烷提取物来制备纳米结构脂质载体（NLC）。制备的NLC颗粒较小，提取物负载的NLC粒径范围为142.9±0.4纳米，GO负载的NLC粒径范围为137.1±0.5纳米，两种制剂的粒径分布均较窄（PI<0.1）。提取物负载的NLC制剂的释放曲线略高于GO负载的NLC制剂。然而，由于NLC颗粒中γ-谷维素的含量较少，它们不符合Higuchi模型。
