Massarolo Kelly Cristina, Denardi de Souza Taiana, Collazzo Carolina Carvalho, Badiale Furlong Eliana, Souza Soares Leonor Almeida de
Laboratory for Mycotoxins and Food Science, Post Graduate Program in Engineering and Food Science, School of Chemistry and Food, Federal University of Rio Grande - FURG, Italy Avenue 8 km, Campus Carreiros, 96203-900 Rio Grande, RS, Brazil.
Laboratory for Mycotoxins and Food Science, Post Graduate Program in Engineering and Food Science, School of Chemistry and Food, Federal University of Rio Grande - FURG, Italy Avenue 8 km, Campus Carreiros, 96203-900 Rio Grande, RS, Brazil.
Food Chem. 2017 Aug 1;228:43-49. doi: 10.1016/j.foodchem.2017.01.127. Epub 2017 Jan 28.
This study evaluated the effect of the solid state cultivation (SSC) time of rice bran by Rhizopus oryzae on γ-oryzanol recovery and its antioxidant properties. Gamma-oryzanol was extracted with organic solvents and its extracts were characterized by GC-FID and HPLC-UV. The antioxidant capacity was assessed by DPPH and ABTS assays, β-carotene/linoleic acid system, and reduction of oxidation in lipid system. The biomass showed the γ-oryzanol recovery increased by 51.5% (20.52mg/g), and 5.7% in polyunsaturated fatty acids. The γ-oryzanol major components changing in their profile. The γ-oryzanol extract from biomass (72h) showed the greatest DPPH inhibition (59.0%), while 90.5% inhibition of oxidation of β-carotene/linoleic acid system, and 30% reduction of the indicators of oxidation in olive oil was observed in the one cultivated at 96h, these behaviors were confirmed by PCA analyses. SSC provides an increase in the γ-oryzanol recovery followed by improving of the functional properties of rice bran.
本研究评估了米根霉对米糠进行固态培养（SSC）的时间对γ-谷维素回收率及其抗氧化性能的影响。用有机溶剂提取γ-谷维素，并用气相色谱-火焰离子化检测器（GC-FID）和高效液相色谱-紫外检测器（HPLC-UV）对其提取物进行表征。通过DPPH和ABTS测定、β-胡萝卜素/亚油酸体系以及脂质体系中氧化的减少来评估抗氧化能力。生物量显示γ-谷维素回收率提高了51.5%（20.52mg/g），多不饱和脂肪酸提高了5.7%。γ-谷维素的主要成分在其分布上发生了变化。来自生物量（72小时）的γ-谷维素提取物表现出最大的DPPH抑制率（59.0%），而在96小时培养的样品中观察到β-胡萝卜素/亚油酸体系氧化抑制率为90.5%，橄榄油氧化指标降低了30%，这些行为通过主成分分析（PCA）得到了证实。固态培养提高了γ-谷维素的回收率，随后改善了米糠的功能特性。
