Eh Suk Vicit Rizal, Mohd Latif Farhanim, Teo Yin Yin, Misran Misni
Colloid Laboratory, Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
J Food Sci Technol. 2020 Sep;57(9):3259-3266. doi: 10.1007/s13197-020-04357-x. Epub 2020 Mar 23.
Lipid nanocarrier displays the advantages over conventional drug carriers as they are formulated with biodegradable and non-irritant lipids. However, the main drawbacks are the agglomeration of lipid particles, instability over storage, low drug loading, and the burst release of active ingredients. In this study, we investigated the effects of various polysorbate nonionic surfactants namely Tween 20, 40, 60, or 80 on the nanostructured lipid carrier (NLC). NLC incorporated with polysorbate nonionic surfactant was prepared by using high-pressure homogenization technique. The average size was reduced to 139.9 ± 15.8 nm in the presence of Tween 80 and remained stable in nano-size even incubated for 28 days. Encapsulation of l-ascorbic acid or Gold Tri.E 30 showed a high encapsulation efficiency of more than 75%, where the highest was Gold Tri.E in the presence of Tween 60 at 99.7%. In vitro release study showed that the release of both l-ascorbic acid and Gold Tri.E was significantly reduced in NLC with Tween as compared to bare active ingredients and NLC without Tween. In conclusion, the incorporation of Tween successfully produced a lipid nanocarrier that has the potential to be developed as a carrier of various active ingredients such as nutrients, extracts, and drugs.
脂质纳米载体相较于传统药物载体具有优势，因为它们是由可生物降解且无刺激性的脂质制成。然而，其主要缺点是脂质颗粒的聚集、储存时的不稳定性、低载药量以及活性成分的突释。在本研究中，我们研究了各种聚山梨醇酯非离子表面活性剂，即吐温20、40、60或80对纳米结构脂质载体（NLC）的影响。通过高压均质技术制备了含有聚山梨醇酯非离子表面活性剂的NLC。在吐温80存在的情况下，平均粒径减小至139.9±15.8纳米，即使孵育28天仍保持纳米尺寸的稳定性。l-抗坏血酸或Gold Tri.E 30的包封率显示超过75%，其中在吐温60存在的情况下，Gold Tri.E的包封率最高，为99.7%。体外释放研究表明，与未添加吐温的裸活性成分和NLC相比，添加吐温的NLC中l-抗坏血酸和Gold Tri.E的释放均显著降低。总之，吐温的加入成功制备了一种脂质纳米载体，它有潜力被开发成各种活性成分如营养物质、提取物和药物的载体。
