Lin Yuanfeng, Xie Xinlei, Yuan Bo, Fu Jie, Liu Linyi, Tian Hua, Chen Tao, He Dongping
College of Food Science and Engineering, Wuhan polytechnic University.
CABIO Bioengineering (Wuhan) Co., Ltd.
J Oleo Sci. 2018 Feb 1;67(2):215-224. doi: 10.5650/jos.ess17166. Epub 2018 Jan 23.
This study is aimed to explore the optimal conditions of cell disruption in the extraction algae oil process, using alkaline protease to disrupt cell of Schizochytrium sp. to extract oil in this paper. The effects of enzymatic lysis temperature, enzymatic lysis time, enzyme dosage and pH value on oil yield and DHA yield were studied. Through the combination of single factor test and response surface design, the optimal cell disruption conditions were screened out. The fatty acid composition of algal oil was analyzed by gas chromatography-massspectrometry (GC-MS). The results showed that when the conditions were: enzymatic lysis temperature 55°C, enzymatic lysis time 9 h, enzyme dosage 3% of biomass and pH 8,oil yield and DHA yield reached the highest 14.52 g/L and 7.12 g/L, respectively. When the strains were cultured in 50 L fermentor, oil yield reached 26.27 g/L and DHA yield reached 12.89 g/L. They were 1.81 times higher than that in shake-flask cultivation. The optimization experiment provides the basis for the industrial production of Schizochytrium sp.
本研究旨在探索裂殖壶菌提取藻油过程中细胞破碎的最佳条件，采用碱性蛋白酶对裂殖壶菌细胞进行破碎以提取藻油。研究了酶解温度、酶解时间、酶用量和pH值对油脂得率和DHA得率的影响。通过单因素试验和响应面设计相结合的方法，筛选出最佳的细胞破碎条件。采用气相色谱-质谱联用仪（GC-MS）分析藻油的脂肪酸组成。结果表明，当条件为：酶解温度55℃、酶解时间9 h、酶用量为生物量的3%、pH值为8时，油脂得率和DHA得率分别达到最高值14.52 g/L和7.12 g/L。当菌株在50 L发酵罐中培养时，油脂得率达到26.27 g/L，DHA得率达到12.89 g/L。它们比摇瓶培养时高出1.81倍。该优化实验为裂殖壶菌的工业化生产提供了依据。
