伊朗人兽共患皮肤利什曼病主要传播媒介——[具体媒介名称未给出]的饲养与生物学特性

Rearing and Biology of , the Main Vector of Anthroponotic Cutaneous Leishmaniasis in Iran.

作者信息

Veysi Arshad, Yaghoobi-Ershadi Mohamad Reza, Rassi Yavar, Hosseini-Vasoukolaei Nasibeh, Jeddi-Tehrani Mahmood, Rezaee-Node Aref, Gholampour Fatemeh, Saeidi Zahra, Fatemi Mahboubeh, Arandian Mohamad Hossein, Khamesipour Ali, Akhavan Amir Ahmad

机构信息

Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Department of Medical Entomology and Vector Control, Health Sciences Research Center, Faculty of Health, Mazandaran University of Medical Sciences, Sari, Iran.

出版信息

J Arthropod Borne Dis. 2017 Dec 30;11(4):504-514. eCollection 2017 Dec.

PMID:29367927

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5775157/

Abstract

BACKGROUND

Establishment of sand flies laboratory colonies is essential to understand various biological aspects of Phlebotominae sand flies. The aims of the current study were to establish the colony of Parrot (1917), the main vector of anthroponotic cutaneous leishmaniasis in old world, and to study biological parameters of this species.

METHODS

The sand flies were reared at 26-28 °C temperature, 14:10 (light: dark) photoperiod and 70-80% relative humidity. Larval diet was a composted mixture of rabbit faces and rabbit pellets which is prepared through a special process. First to fifth generations of were used to define biological parameters.

RESULTS

Results showed that, blood feeding percentage were 42% on chicken, 21% on BALB/c and 37% on golden hamster. Average time of blood digestion, egg incubation, 1 instar larva, pupa and adult emerging was recorded at 3.4, 8.7, 15, 33.3 and 41.2 days after blood feeding, respectively. Mean number of laid eggs was 55.1 and retained eggs were 35 per a female. Fecundity and production rate were 61.6%, and 42.2% respectively. Average longevity recorded at 15.2 days for females and 14.8 days for males.

CONCLUSION

Colony of . has been established for the first time in Iran. Average interval time from egg to adult of this species was 32.5 days. Chicken and golden hamster were recommended as a blood source for colony initiation and routine blood feeding, respectively.

摘要

背景

建立白蛉实验室种群对于了解白蛉亚科白蛉的各种生物学特性至关重要。本研究的目的是建立旧大陆皮肤利什曼病主要传播媒介鹦鹉白蛉（1917）的种群，并研究该物种的生物学参数。

方法

白蛉在温度26 - 28°C、光周期14:10（光照:黑暗）和相对湿度70 - 80%的条件下饲养。幼虫饲料是通过特殊工艺制备的兔脸和兔颗粒的混合堆肥。使用第一代至第五代来确定生物学参数。

结果

结果表明，以鸡为血源时吸血率为42%，以BALB/c小鼠为血源时为21%，以金黄仓鼠为血源时为37%。分别记录了吸血后血液消化、卵孵化、一龄幼虫、蛹和成虫羽化的平均时间为3.4天、8.7天、15天、33.3天和41.2天。每只雌虫平均产卵数为55.1枚，留存卵数为35枚。繁殖力和生产率分别为61.6%和42.2%。记录的雌虫平均寿命为15.2天，雄虫为14.8天。