在实验室条件下评估卵巢附属腺体以确定人兽共患皮肤利什曼病的传播媒介的胎次。
Assessing the Ovarian Accessory Glands to Determine the Parity of , Vector of Zoonotic Cutaneous Leishmaniasis, under Laboratory Condition.
作者信息
Fatemi Mahboubeh, Yaghoobi-Ershadi Mohammad Reza, Mohebali Mehdi, Saeidi Zahra, Veysi Arshad, Khamesipour Ali, Akhavan Amir Ahmad
机构信息
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
J Arthropod Borne Dis. 2017 Mar 14;11(1):161-165. eCollection 2017 Mar.
BACKGROUND
Zoonotic cutaneous leishmaniasis (ZCL) is a neglected tropical disease prevailed in many rural areas of 17 out of 31 provinces in Iran. The main vector of the disease is and the causative agent is in ZCL foci of Iran. In the current study we investigated the validity of accessory glands secretions as an indicator to recognize parous from nulliparous females under laboratory conditions.
METHODS
Over 235 laboratory-reared females of were dissected in 6 groups including: newly emerged, one hour, one day, two days, and three days after blood feeding and also after oviposition under stereo microscope for their parity in 2014-2015.
RESULTS
Transparent glands were compatible with nulliparous only in newly emerged sand flies. In sand flies dissected after oviposition, accessory glands were rather large as a result of oviposition though they were transparent.
CONCLUSION
The accessory glands secretions could not be as an indicator for distinguishing parous from nulliparous of females.
背景
人兽共患皮肤利什曼病（ZCL）是一种被忽视的热带疾病，在伊朗31个省中的17个省的许多农村地区流行。该病的主要传播媒介是 ，在伊朗ZCL疫源地的病原体是 。在本研究中，我们调查了在实验室条件下，副腺分泌物作为识别已产卵和未产卵 雌性个体指标的有效性。
方法
2014年至2015年期间，在体视显微镜下，将235只以上实验室饲养的 雌性个体分为6组进行解剖，包括：新羽化的、吸血后1小时、1天、2天、3天以及产卵后的个体，以确定其生育状态。
结果
透明腺体仅在新羽化的白蛉中与未产卵状态相符。在产卵后解剖的白蛉中，尽管副腺是透明的，但由于产卵的原因，副腺相当大。
结论
副腺分泌物不能作为区分 雌性已产卵和未产卵状态的指标。
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