利用慢病毒载体进行滋养层特异性基因操作。
Trophoblast-specific Genetic Manipulation Using Lentiviral Delivery.
作者信息
Chakraborty Damayanti, Muto Masanaga, Soares Michael J
机构信息
Institute for Reproductive Health and Regenerative Medicine, Departments of Pathology & Laboratory Medicine and Pediatrics, University of Kansas Medical Center, Kansas City, KS, USA.
Fetal Health Research, Children's Research Institute, Children's Mercy, Kansas City, MO, USA.
出版信息
Bio Protoc. 2017 Dec 20;7(24). doi: 10.21769/BioProtoc.2652.
Abstract
In this protocol report, we describe a lentiviral gene delivery technique for genetic modification of the rat trophoblast cell lineage. Lentiviral packaged gene constructs can be efficiently and specifically delivered to the trophoblast cell lineage of the blastocyst. The consequences of 'gain-of-function' and 'loss-of-function' blastocyst manipulations can be evaluated with outgrowth assays or following transfer to pseudopregnant rats.
摘要
在本方案报告中，我们描述了一种用于大鼠滋养层细胞系基因改造的慢病毒基因递送技术。慢病毒包装的基因构建体可以高效且特异性地递送至囊胚的滋养层细胞系。通过体外生长试验或转移至假孕大鼠后，可以评估“功能获得”和“功能丧失”的囊胚操作的后果。
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2
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4
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