使用xCELLigence RTCA仪器测量细胞黏附。
Using xCELLigence RTCA Instrument to Measure Cell Adhesion.
作者信息
Hamidi Hellyeh, Lilja Johanna, Ivaska Johanna
机构信息
Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
Department of Biochemistry, University of Turku, Turku, Finland.
出版信息
Bio Protoc. 2017 Dec 20;7(24). doi: 10.21769/BioProtoc.2646.
Abstract
Cell adhesion to neighbouring cells and to the underlying extracellular matrix (ECM) is a fundamental requirement for the existence of multicellular organisms. As such, the formation, stability and dissociation of cell adhesions are subject to tight control in space and time and perturbations within the sophisticated adhesion machinery are associated with a variety of human pathologies. Here, we outline a simple protocol to monitor alterations in cell adhesion to the ECM, for example, following genetic manipulations or overexpression of a protein of interest or in response to drug treatment, using the xCELLigence real-time cell analysis (RTCA) system.
摘要
细胞与相邻细胞以及细胞外基质（ECM）的粘附是多细胞生物生存的基本条件。因此，细胞粘附的形成、稳定性和解离在空间和时间上受到严格控制，复杂粘附机制内的扰动与多种人类疾病相关。在此，我们概述了一种简单的方案，用于使用xCELLigence实时细胞分析（RTCA）系统监测细胞与ECM粘附的变化，例如在基因操作、感兴趣蛋白质的过表达之后，或在药物治疗反应中。
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