Yermishin A P, Svitoch O V, Voronkova E V, Gukasian O N, Luksha V I
Genetika. 2016 May;52(5):569-78.
The allelic dosage of disease and pest resistance genes was determined in 11 prospective potato varieties and hybrids by means of detecting the corresponding PCR DNA markers in their progeny from crosses with specially selected testers. It was revealed that most (65%) resistance genes in the analyzed parental lines were present as a single dominant allele (simplex). Nevertheless, we were able to find some multiplex lines valuable for breeding. The Yanka variety and the clone 52-03-16 had one triplex and one duplex of resistance genes, the Lilea and Charaunik varieties and the clone 106-04-17 had two resistance genes in duplex, and the Uladar and Falvarak varieties and the clone 45-04-24 were duplex for some single genes. The highest number of multiplex lines was detected for the genes Ry sto , H1, and Sen1. Only simplex genotypes were revealed for the Gro-1-4 and PLRV1 genes.
通过检测11个预期马铃薯品种和杂交种与特别选择的测试品种杂交后代中的相应PCR DNA标记，确定了抗病虫基因的等位基因剂量。结果表明，分析的亲本系中大多数（65%）抗性基因以单一显性等位基因（单式）形式存在。然而，我们能够找到一些对育种有价值的多基因系。扬卡品种和克隆52-03-16有一个抗性基因三联体和一个抗性基因双链体，利莱亚和查劳尼克品种以及克隆106-04-17有两个抗性基因双链体，乌拉达尔和法尔瓦拉克品种以及克隆45-04-24的一些单个基因是双链体。Ry sto、H1和Sen1基因检测到的多基因系数量最多。Gro-1-4和PLRV1基因仅表现为单式基因型。
