Arthur R P, Mason D
J Exp Med. 1986 Apr 1;163(4):774-86. doi: 10.1084/jem.163.4.774.
An mAb MRC OX-22, reactive with the high molecular weight forms of the rat leukocyte-common antigen, has revealed a heterogeneity among CD4+ T cells in this species. Approximately two-thirds are CD4+, OX-22+, and one-third are CD4+, OX-22-. This phenotypic heterogeneity was found to be associated with a functional one. CD4+, OX-22+ cells proliferated well in mixed leukocyte culture, responded to the T cell mitogen Con A, and produced IL-2 on activation. In contrast, the CD4+, OX-22- cells performed poorly in these assays, but unlike CD4+, OX-22+ cells, did provide effective help for B cells. By sampling supernatants from cultures containing primed B cells and either of the two CD4+ T cell subsets, it was shown that, when specific antigen was included in the cultures, those containing the OX-22- subset of CD4+ cells produced high levels of antibody and some IL-2, whereas those containing the OX-22+ cells produced neither. In contrast, when specific antigen was replaced by Con A, the B cell cultures supplemented with CD4+, OX-22+ cells synthesized much higher levels of IL-2 than those containing CD4+, OX-22- cells, but only the latter cultures produced detectable levels of antibody. The data show that inducer/helper T cells comprise two functional subsets: one that, on appropriate stimulation, synthesizes high levels of IL-2, and may therefore be presumed to play an important role in cell-mediated immunity, and another that plays an essential role in humoral responses to soluble antigens. The significance of this functional heterogeneity, with regard to the possible independent regulation of cellular and humoral responses, is briefly considered.
一种与大鼠白细胞共同抗原的高分子量形式发生反应的单克隆抗体MRC OX - 22，揭示了该物种CD4⁺ T细胞之间的异质性。大约三分之二是CD4⁺、OX - 22⁺，三分之一是CD4⁺、OX - 22⁻。发现这种表型异质性与功能异质性相关。CD4⁺、OX - 22⁺细胞在混合白细胞培养中增殖良好，对T细胞有丝分裂原刀豆蛋白A有反应，并在激活时产生白细胞介素 - 2。相比之下，CD4⁺、OX - 22⁻细胞在这些试验中表现不佳，但与CD4⁺、OX - 22⁺细胞不同，确实能为B细胞提供有效的辅助。通过从含有致敏B细胞和两种CD4⁺ T细胞亚群之一的培养物中采集上清液，结果表明，当培养物中包含特异性抗原时，含有CD4⁺细胞的OX - 22⁻亚群的培养物产生高水平的抗体和一些白细胞介素 - 2，而含有OX - 22⁺细胞的培养物则两者均不产生。相反，当特异性抗原被刀豆蛋白A取代时，补充有CD4⁺、OX - 22⁺细胞的B细胞培养物合成的白细胞介素 - 2水平比含有CD4⁺、OX - 22⁻细胞的培养物高得多，但只有后者培养物产生可检测水平的抗体。数据表明，诱导/辅助性T细胞包括两个功能亚群：一个在适当刺激下合成高水平的白细胞介素 - 2，因此可能被认为在细胞介导的免疫中起重要作用，另一个在对可溶性抗原的体液反应中起关键作用。简要考虑了这种功能异质性对于细胞和体液反应可能的独立调节的意义。
