Department of Dermatology and Allergology, Charité - Universitätsmedizin Berlin, Berlin, Germany.
Leibniz-Institute of Vegetable and Ornamental Crops, Grossbeeren, Germany.
Clin Exp Allergy. 2018 Apr;48(4):424-432. doi: 10.1111/cea.13099. Epub 2018 Feb 28.
Celery (Apium graveolens L.) is a vegetable consumed world-wide. Celery stalks and celeriac roots are often ingredients in convenient food products like spice blends and soups.
In this study, we examined the allergenicity of distinct celeriac cultivars.
Sixteen celery-allergic patients were identified using a double-blind, placebo-controlled food challenge. Ten different celeriac cultivars were used for skin prick testing in the patients. Two cultivars were further applied for oral food challenges; their protein composition was analysed by immunoblotting, and contents of major allergen Api g 1 were quantified.
From the 10 investigated celeriac cultivars, two cultivars elicited significantly different skin reactivity ("Anita": 5.0 [2.0-12.0] mm vs "Prinz": 7.0 [3.0-9.5] mm; P = .047). Moreover, "Anita" induced fewer symptoms after a controlled oral-celeriac challenge in 14 patient (P < .001). The protein profiles on 2DE protein gels showed distinct protein patterns and higher protein amounts of Api g 1 in "Prinz" than in "Anita."
Taken together, the data from this study suggest that cultivar Anita is better tolerated in celery-allergic patients than "Prinz." Differences in the protein expression profile between the cultivars, particularly the different content of Api g 1, could cause the different allergenicity.
芹菜（Apium graveolens L.）是一种在世界范围内食用的蔬菜。芹菜茎和块根通常是方便食品如香料混合物和汤的成分。
本研究检测了不同的块根芹品种的变应原性。
通过双盲、安慰剂对照食物挑战确定了 16 名芹菜过敏患者。对 10 种不同的块根芹品种进行了患者的皮肤点刺试验。进一步对两种品种进行了口服食物挑战；用免疫印迹法分析其蛋白质组成，并定量主要过敏原 Api g 1 的含量。
在所研究的 10 种块根芹品种中，有两种品种的皮肤反应明显不同（“Anita”：5.0 [2.0-12.0] mm 对“Prinz”：7.0 [3.0-9.5] mm；P =.047）。此外，在 14 名患者中，“Anita”进行控制口服块根芹挑战后引起的症状较少（P <.001）。2-DE 蛋白质凝胶上的蛋白质图谱显示，“Prinz”中的 Api g 1 蛋白表达模式明显不同，且蛋白含量更高。
总的来说，本研究的数据表明，在芹菜过敏患者中，品种 Anita 比“Prinz”更耐受。品种之间的蛋白质表达谱差异，特别是 Api g 1 的含量不同，可能导致不同的变应原性。
