Guo Li-Na, Li Ying, Hsueh Po-Ren, Wang Peng, Zhao Yu-Pei, Xu Ying-Chun
Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
Departments of Laboratory Medicine and Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan.
PLoS One. 2018 Jan 25;13(1):e0191715. doi: 10.1371/journal.pone.0191715. eCollection 2018.
Infectious endocarditis (IE) can be caused by various pathogens, from dominating agents such as viridans group streptococci and staphylococci to rare species that are less virulent and not typically considered to be pathogens. In this study, we have isolated a novel species from a patient with problem of IE which was genetically most closely related to 'Bergeyella cardium', a causative pathogen of IE first reported in Korea in 2015 as a new species of the genus Bergeyella, with a similarity of 98.8% in 16S rRNA sequences. Microbiological characteristics, including morphology, biochemical identification and antimicrobial susceptibility profiling, of this novel species were determined. This fastidious Gram-negative bacillus could only be identified successfully by molecular sequencing analysis at present, and it exhibited low minimum inhibitory concentrations to the antibiotics tested except for aminoglycosides. Phylogeny analysis revealed this novel species clustered well with 'B. cardium' and other close species of genus Bergeyella.
感染性心内膜炎（IE）可由多种病原体引起，从主要病原体如草绿色链球菌和葡萄球菌到毒力较弱且通常不被视为病原体的罕见菌种。在本研究中，我们从一名患有IE问题的患者中分离出一种新菌种，该菌种在基因上与“心伯格菌（Bergeyella cardium）”关系最为密切，“心伯格菌”是2015年在韩国首次报道的一种IE致病病原体，为伯格菌属的一个新物种，其16S rRNA序列相似度为98.8%。我们确定了该新菌种的微生物学特征，包括形态、生化鉴定和抗菌药敏谱。这种苛求的革兰氏阴性杆菌目前只能通过分子测序分析成功鉴定，除氨基糖苷类抗生素外，它对所测试的抗生素表现出较低的最低抑菌浓度。系统发育分析表明，该新菌种与“心伯格菌”以及伯格菌属的其他近缘菌种聚类良好。
