Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China.
BMC Microbiol. 2020 Feb 12;20(1):32. doi: 10.1186/s12866-020-1715-0.
Bergeyella cardium infection is becoming increasingly prevalent in patients with infective endocarditis, suggesting its significance in disease pathogenesis. However, few studies have fully characterized this species.
Herein, we report the morphological and physiological characteristics, as well as whole genome sequencing of a newly identified B. cardium HPQL strain isolated from a patient with infective endocarditis. Results from the cellular morphology and biochemical analysis provide basic knowledge on the new pathogen. The whole genome sequencing of B. cardium HPQL consists of a circular chromosome with a total length of 2,036,890 bp. No plasmid was detected. Comparative genomics were carried out then. Antibiotics resistance related genes, pathogenesis related genes, predicted insertion sequences, genome islands and predicted CRISPRs sequences were demonstrated. To our knowledge, this is the first study to provide a complete genome sequence for Bergeyella spp.
This study provides fundamental phenotypic and genomic information for the newly identified fastidious infective endocarditis causative bacteria, B. cardium. Our results provide insights into effective clinical diagnosis and treatment of this pathogen.
贝氏考克斯菌感染在感染性心内膜炎患者中越来越普遍，这表明其在疾病发病机制中的重要性。然而，很少有研究能充分描述这种物种。
本研究报告了从感染性心内膜炎患者中分离出的一株新鉴定的贝氏考克斯菌 HPQL 菌株的形态和生理特征以及全基因组测序结果。细胞形态和生化分析的结果为新病原体提供了基础知识。贝氏考克斯菌 HPQL 的全基因组测序由一个总长度为 2,036,890bp 的圆形染色体组成。未检测到质粒。然后进行了比较基因组学分析。展示了抗生素耐药相关基因、发病机制相关基因、预测插入序列、基因组岛和预测的 CRISPRs 序列。据我们所知，这是首次对贝氏菌属提供完整的基因组序列。
本研究为新鉴定的苛养性感染性心内膜炎病原体贝氏考克斯菌提供了基本的表型和基因组信息。我们的研究结果为该病原体的有效临床诊断和治疗提供了思路。
