Department of Pathological Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo 113-8510, Japan.
Mol Cells. 2018 Jan 31;41(1):50-54. doi: 10.14348/molcells.2018.2215. Epub 2018 Jan 23.
Atg5 and Atg7 have long been considered as essential molecules for autophagy. However, we found that cells lacking these molecules still form autophagic vacuoles and perform autophagic protein degradation when subjected to certain stressors. During this unconventional autophagy pathway, autophagosomes appeared to be generated in a Rab9-dependent manner by the fusion of vesicles derived from the -Golgi and late endosomes. Therefore, mammalian autophagy can occur via at least two different pathways; the Atg5/Atg7-dependent conventional pathway and an Atg5/Atg7-independent alternative pathway.
Atg5 和 Atg7 长期以来一直被认为是自噬的必需分子。然而，我们发现，当细胞受到某些应激源时，即使缺乏这些分子，仍然会形成自噬小泡并进行自噬蛋白降解。在这种非经典的自噬途径中，自噬体似乎通过源自 -高尔基体和晚期内体的小泡融合，以 Rab9 依赖性的方式产生。因此，哺乳动物自噬至少可以通过两种不同的途径发生：依赖 Atg5/Atg7 的经典途径和不依赖 Atg5/Atg7 的替代途径。
