a Department of Chemical and Petroleum Engineering , Sharif University of Technology , Tehran , Iran.
J Biomater Sci Polym Ed. 2018 Apr;29(6):683-700. doi: 10.1080/09205063.2018.1433422. Epub 2018 Feb 2.
Cartilage is an avascular tissue with limited self-repair ability. Since the methods for treatment of cartilage defects have not been effective, new therapies based on tissue engineering are considered over the recent years. In this study, human cartilage tissue was decellularized and porous injectable microcarriers (MCs) composed of acellular extracellular matrix (ECM) of cartilage tissue and chitosan (CS), with different ECM weight ratios, were fabricated by electrospraying technique to be used in the treatment of articular cartilage defects. Various properties of ECM/CS MCs such as microstructure, mechanical strength, water uptake behaviour, and biodegradability rate were investigated. MCs with 1% ECM and 2% CS show appropriate characteristics in terms of pore size, density, porosity, and mechanical properties. MTT cytotoxicity assays performed on chondrocyte cells cultured on ECM/CS MCs with various amounts of ECM showed that the sample with 1% ECM content had the greatest cell attachment. Overall, it can be concluded that the prepared MCs with 1% ECM could be a potential candidate to be used in cartilage tissue engineering applications.
软骨是一种无血管组织，自我修复能力有限。由于治疗软骨缺损的方法并不有效，近年来，人们开始考虑基于组织工程的新疗法。在这项研究中，通过脱细胞技术处理人软骨组织，并采用静电纺丝技术制备了由脱细胞软骨组织细胞外基质（ECM）和壳聚糖（CS）组成、具有不同 ECM 重量比的多孔可注射微载体（MCs），用于治疗关节软骨缺损。研究了 ECM/CS MCs 的各种性质，如微观结构、机械强度、吸水率和生物降解率。ECM/CS MCs 的 ECM 含量为 1%、CS 含量为 2%时，在孔径、密度、孔隙率和机械性能方面具有适当的特性。对在含有不同量 ECM 的 ECM/CS MCs 上培养的软骨细胞进行 MTT 细胞毒性试验表明，含有 1% ECM 的样品具有最大的细胞附着性。总的来说，制备的含有 1% ECM 的 MCs 可能是软骨组织工程应用的潜在候选材料。
