Park Joon Yeong, Song Bo Ram, Lee Jin Woo, Park Seung Hun, Kang Tae Woong, Yun Hee-Woong, Park Sang-Hyug, Min Byoung Hyun, Kim Moon Suk
Department of Molecular Science and Technology, Ajou University, Suwon 16499, Korea.
Cell Therapy Center, Ajou University Medical Center, Suwon 16499, Korea.
Polymers (Basel). 2019 Feb 2;11(2):247. doi: 10.3390/polym11020247.
In this paper, a cartilage acellular-matrix (CAM) is chosen as a biomaterial for an effective antiadhesive barrier to apply between injured tissue and healthy tissues or organs. CAM is cross-linked using glutaraldehyde to create a cross-linked CAM (Cx-CAM) film. Cx-CAM has higher elastic modulus and toughness and more hydrophobic surface properties than CAM before cross-linking. Small intestinal submucosa (SIS), cross-linked SIS (Cx-SIS) as a negative control, and Seprafilm as a positive control are used in an experiment as adhesion barriers. Human umbilical vein endothelial cells (HUVECs) on SIS, Cx-SIS, or in a culture plate get attached and effectively proliferate for 7 days, but Cx-CAM and Seprafilm allow for little or no attachment and proliferation of HUVECs, thus manifesting antiadhesive and antiproliferative effects. In animals with surgical damage to the peritoneal wall and cecum, Cx-CAM and Seprafilm afford little adhesion and negligible inflammation after seven days, as confirmed by hematoxylin and eosin staining and macrophage staining, in contrast to an untreated-injury model, SIS, or Cx-SIS film. Cx-CAM significantly suppresses the formation of blood vessels between the peritoneal wall and cecum, as confirmed by CD31 staining. Overall, the newly designed Cx-CAM film works well as an antiadhesion barrier and has better anti-tissue adhesion efficiency.
在本文中，选择软骨脱细胞基质（CAM）作为生物材料，用于在受损组织与健康组织或器官之间构建有效的抗粘连屏障。使用戊二醛对CAM进行交联，以制备交联的CAM（Cx-CAM）膜。与交联前的CAM相比，Cx-CAM具有更高的弹性模量和韧性以及更疏水的表面特性。在实验中，将小肠黏膜下层（SIS）、交联的SIS（Cx-SIS）作为阴性对照以及Seprafilm作为阳性对照用作粘连屏障。接种于SIS、Cx-SIS上或培养板中的人脐静脉内皮细胞（HUVECs）能够附着并在7天内有效增殖，但Cx-CAM和Seprafilm几乎不允许HUVECs附着和增殖，从而表现出抗粘连和抗增殖作用。在腹膜壁和盲肠受到手术损伤的动物中，苏木精-伊红染色和巨噬细胞染色证实，与未处理的损伤模型、SIS或Cx-SIS膜相比，Cx-CAM和Seprafilm在7天后几乎没有粘连且炎症可忽略不计。CD31染色证实，Cx-CAM显著抑制腹膜壁和盲肠之间血管的形成。总体而言，新设计的Cx-CAM膜作为抗粘连屏障效果良好，且具有更好的抗组织粘连效率。
