Abdelmoteleb Ali, Troncoso-Rojas Rosalba, Gonzalez-Soto Tania, González-Mendoza Daniel
Instituto de Ciencias Agrícolas de la Universidad Autónoma de Baja California (ICA-UABC), CP 21705, Ejido Nuevo León, Baja California, Mexico.
Botany Department, Faculty of Agriculture, Menoufia University, Shebin El-Kom 32511, Egypt.
Mycobiology. 2017 Dec;45(4):385-391. doi: 10.5941/MYCO.2017.45.4.385. Epub 2017 Dec 31.
The ability of , strain ALICA to produce three mycolytic enzymes (chitinase, β-1,3-glucanase, and protease), was carried out by the chemical standard methods. ALICA was screened based on their antifungal activity in dual plate assay and cell-free culture filtrate (25%) against five different phytopathogenic fungi , sp., , , and . The ALICA detected positive for chitinase, β-1,3-glucanase and protease enzymes. Fungal growth inhibition by both strain ALICA and its cell-free culture filtrate ranged from 51.36% to 86.3% and 38.43% to 68.6%, respectively. Moreover, hyphal morphological changes like damage, broken, swelling, distortions abnormal morphology were observed. Genes expression of protease, β-1,3-glucanase, and lipopeptides (subtilosin and subtilisin) were confirmed their presence in the supernatant of strain ALICA. Our findings indicated that strain ALICA provided a broad spectrum of antifungal activities against various phytopathogenic fungi and may be a potential effective alternative to chemical fungicides.
通过化学标准方法对ALICA菌株产生三种溶菌酶（几丁质酶、β-1,3-葡聚糖酶和蛋白酶）的能力进行了检测。基于其在双平板试验中的抗真菌活性以及对五种不同植物病原真菌（ 种、 种、 种、 种和 种）的无细胞培养滤液（25%）对ALICA进行了筛选。检测发现ALICA菌株的几丁质酶、β-1,3-葡聚糖酶和蛋白酶呈阳性。ALICA菌株及其无细胞培养滤液对真菌生长的抑制率分别为51.36%至86.3%和38.43%至68.6%。此外，还观察到了菌丝形态的变化，如损伤、断裂、肿胀、扭曲和异常形态。蛋白酶、β-1,3-葡聚糖酶和脂肽（枯草菌素和枯草杆菌蛋白酶）的基因表达在ALICA菌株的上清液中得到了证实。我们的研究结果表明，ALICA菌株对多种植物病原真菌具有广谱抗真菌活性，可能是化学杀菌剂的一种潜在有效替代品。
