Su Yu, Shao Chenggang, Huang Xielin, Qi Jinxia, Ge Renshan, Guan Huaqin, Lin Zhenkun
Center of Scientific Research, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China.
The Department of Gastroenterology and Hepatology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 310018, China.
Anal Bioanal Chem. 2018 Mar;410(7):1885-1891. doi: 10.1007/s00216-017-0801-0. Epub 2018 Jan 26.
A new type of magnetic nanoparticles (MNPs), as the absorbents of bisphenol A (BPA), was prepared by functionalization of FeO@SiO with BPA-specific aptamer in this work. ssDNA aptamer was immobilized on the FeO@SiO surface through biotin-avidin interactions, playing a role of the specific probe for BPA. The resultant materials (Apt-MNPs) exhibited outstanding magnetic responsibility and can be separated efficiently by the magnetic field. Experimental results also showed that Apt-MNPs had large adsorption capacity and high competitive selectivity for the targeted compound BPA. Furthermore, Apt-MNPs were adopted as the specific absorbents to extract and enrich BPA from human serum and urine samples. Therefore, an efficient detection method of BPA was developed in combination with high-performance liquid chromatography (HPLC). The linearity of the method was over a range of 5-10,000 ng mL with a correlation coefficient of 0.99997, and the limit of detections (LODs) for serum and urine were 2.0 and 1.0 ng mL, respectively. The recoveries of BPA in the spiked human serum and urine samples were 90.8 ± 7.3% (RSD) and 92.3 ± 1.5%, respectively. Our results demonstrated that Apt-MNPs were high-performance adsorbents for extracting and enriching BPA, resulting in fast and efficient detection of BPA in serum and urine samples. Graphical abstract Aptamer-MNPs were effective for BPA separation from serum and urine.
在本研究中，通过用双酚A（BPA）特异性适配体对FeO@SiO进行功能化，制备了一种新型磁性纳米颗粒（MNPs）作为双酚A的吸附剂。单链DNA适配体通过生物素-抗生物素蛋白相互作用固定在FeO@SiO表面，起到对双酚A的特异性探针作用。所得材料（Apt-MNPs）表现出出色的磁响应性，可通过磁场高效分离。实验结果还表明，Apt-MNPs对目标化合物双酚A具有较大的吸附容量和高竞争选择性。此外，Apt-MNPs被用作特异性吸附剂，从人血清和尿液样本中提取和富集双酚A。因此，结合高效液相色谱（HPLC）开发了一种双酚A的高效检测方法。该方法的线性范围为5-10000 ng/mL，相关系数为0.99997，血清和尿液的检测限分别为2.0和1.0 ng/mL。加标人血清和尿液样本中双酚A的回收率分别为90.8±7.3%（相对标准偏差）和92.3±1.5%。我们的结果表明，Apt-MNPs是用于提取和富集双酚A的高性能吸附剂，可快速高效地检测血清和尿液样本中的双酚A。图形摘要适配体-MNPs对从血清和尿液中分离双酚A有效。
