Furuta Akira, Yamamoto Tokunori, Suzuki Yasuyuki, Gotoh Momokazu, Egawa Shin, Yoshimura Naoki
Department of Urology, Jikei University School of Medicine, 3-25-8 Nishishinbashi, Minato-ku, Tokyo, 105-8461, Japan.
Department of Urology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Int Urogynecol J. 2018 Jul;29(7):961-966. doi: 10.1007/s00192-017-3547-5. Epub 2018 Jan 25.
Chronic inflammatory conditions seem to be a shared characteristic in patients with interstitial cystitis (IC) and overactive bladder (OAB). Thus, we measured 40 inflammatory urine markers in IC patients with or without Hunner's lesions (HIC and NHIC respectively) and OAB patients.
Urine was collected from consecutive HIC patients, NHIC patients, and age and gender-matched OAB patients with no history of IC, recurrent urinary tract infection or bladder cancer. The diagnosis of IC was based on the Asian IC guideline criteria. A representative 40 inflammatory growth factors, cytokines, and chemokines in urine were measured using a MILLIPLEX immunoassay kit. Statistical differences in these markers among the groups were determined by nonparametric ANOVA followed by multiple comparison test. The diagnostic efficiency of these markers was measured using receiver operating characteristic analysis.
Vascular endothelial growth factor (VEGF), interleukin-1α (IL-1α), IL-6, and chemokines including CCL2, CCL5, CXCL1, CXCL8, and CXCL10 were significantly increased in HIC (n = 30) and NHIC (n = 30) patients compared with OAB (n = 28) patients. The significant increases in CXCL8 and CXCL10 were also found in HIC patients compared with NHIC patients. However, there were no significant differences in the other urine markers among the groups. Area under the curves for VEGF, CXCL10, CXCL8, IL-1α, CCL5, CCL2, IL-6, and CXCL1 to detect IC in these patients were 0.87, 0.86, 0.81, 0.80, 0.80, 0.71, 0.66, and 0.50 respectively.
The increases in angiogenesis-associated proteins such as VEGF and CXCL10 may be pathophysiologically important for the development of IC.
慢性炎症状态似乎是间质性膀胱炎（IC）和膀胱过度活动症（OAB）患者的一个共同特征。因此，我们检测了有或无Hunner溃疡的IC患者（分别为HIC和NHIC）以及OAB患者的40种炎症性尿液标志物。
收集连续的HIC患者、NHIC患者以及年龄和性别匹配且无IC病史、复发性尿路感染或膀胱癌病史的OAB患者的尿液。IC的诊断基于亚洲IC指南标准。使用MILLIPLEX免疫分析试剂盒检测尿液中具有代表性的40种炎症生长因子、细胞因子和趋化因子。通过非参数方差分析和多重比较检验确定这些标志物在各组间的统计学差异。使用受试者工作特征分析评估这些标志物的诊断效能。
与OAB患者（n = 28）相比，HIC患者（n = 30）和NHIC患者（n = 30）中血管内皮生长因子（VEGF）、白细胞介素-1α（IL-1α）、IL-6以及包括CCL2、CCL5、CXCL1、CXCL8和CXCL10在内的趋化因子显著升高。与NHIC患者相比，HIC患者中CXCL8和CXCL10也显著升高。然而，其他尿液标志物在各组间无显著差异。在这些患者中，VEGF、CXCL10、CXCL8、IL-1α、CCL5、CCL2、IL-6和CXCL1检测IC的曲线下面积分别为0.87、0.86、0.81、0.80、0.80、0.71、0.66和0.50。
VEGF和CXCL10等与血管生成相关的蛋白质增加可能在IC的发病机制中具有重要意义。
