使用细胞分选微流控技术从诊断为克隆性浆细胞疾病的患者血液中分离循环浆细胞。
Isolation of circulating plasma cells from blood of patients diagnosed with clonal plasma cell disorders using cell selection microfluidics.
作者信息
Kamande Joyce W, Lindell Maria A M, Witek Małgorzata A, Voorhees Peter M, Soper Steven A
机构信息
Department of Biomedical Engineering, The University of North Carolina at Chapel Hill, NC 27599, USA.
出版信息
Integr Biol (Camb). 2018 Feb 19;10(2):82-91. doi: 10.1039/c7ib00183e.
Blood samples from patients with plasma cell disorders were analysed for the presence of circulating plasma cells (CPCs) using a microfluidic device modified with monoclonal anti-CD138 antibodies. CPCs were immuno-phenotyped using a CD38/CD56/CD45 panel and identified in 78% of patients with monoclonal gammopathy of undetermined significance (MGUS), all patients with smouldering and symptomatic multiple myeloma (MM), and none in the controls. The burden of CPCs was higher in patients with symptomatic MM compared with MGUS and smouldering MM (p < 0.05). FISH analysis revealed the presence of chromosome 13 deletions in CPCs that correlated with bone marrow results. Point mutations in KRAS were identified, including different mutations from sub-clones derived from the same patient. The microfluidic assay represents a highly sensitive method for enumerating CPCs and allows for the cytogenetic and molecular characterization of CPCs.
使用用单克隆抗CD138抗体修饰的微流控装置，对浆细胞疾病患者的血样进行循环浆细胞（CPC）检测。使用CD38/CD56/CD45组合对CPC进行免疫表型分析，在78%意义未明的单克隆丙种球蛋白病（MGUS）患者、所有冒烟型和有症状的多发性骨髓瘤（MM）患者中检测到CPC，而在对照组中未检测到。有症状的MM患者的CPC负荷高于MGUS和冒烟型MM患者（p<0.05）。荧光原位杂交（FISH）分析显示CPC中存在13号染色体缺失，这与骨髓结果相关。鉴定出KRAS中的点突变，包括来自同一患者亚克隆的不同突变。微流控检测是一种用于计数CPC的高度灵敏方法，并且能够对CPC进行细胞遗传学和分子特征分析。
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