Jackson Joshua M, Witek Małgorzata A, Kamande Joyce W, Soper Steven A
Department of Chemistry, University of Kansas, USA.
Chem Soc Rev. 2017 Jul 17;46(14):4245-4280. doi: 10.1039/c7cs00016b.
We present a critical review of microfluidic technologies and material effects on the analyses of circulating tumour cells (CTCs) selected from the peripheral blood of cancer patients. CTCs are a minimally invasive source of clinical information that can be used to prognose patient outcome, monitor minimal residual disease, assess tumour resistance to therapeutic agents, and potentially screen individuals for the early diagnosis of cancer. The performance of CTC isolation technologies depends on microfluidic architectures, the underlying principles of isolation, and the choice of materials. We present a critical review of the fundamental principles used in these technologies and discuss their performance. We also give context to how CTC isolation technologies enable downstream analysis of selected CTCs in terms of detecting genetic mutations and gene expression that could be used to gain information that may affect patient outcome.
我们对微流控技术以及材料对从癌症患者外周血中筛选循环肿瘤细胞（CTC）分析的影响进行了批判性综述。CTC是一种微创临床信息来源，可用于预测患者预后、监测微小残留病、评估肿瘤对治疗药物的耐药性，并有可能用于筛查个体以实现癌症的早期诊断。CTC分离技术的性能取决于微流控结构、分离的基本原理以及材料的选择。我们对这些技术所使用的基本原理进行了批判性综述，并讨论了它们的性能。我们还阐述了CTC分离技术如何在检测可用于获取可能影响患者预后信息的基因突变和基因表达方面，实现对所选CTC的下游分析。
