Chen Jiao, Shi Hao
College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China.
Se Pu. 2017 Dec 8;35(12):1229-1239. doi: 10.3724/SP.J.1123.2017.09017.
Chiral separation is important in biological medicine and other fields. High performance liquid chromatography (HPLC) is widely used in chiral separation and analysis for its economic, rapid and efficient characteristics. Chiral stationary phase (CSP) is the key to achieve chiral resolution in HPLC. Meanwhile, the key to preparing effective CSP is the screening of chiral selector. In recent years, a lot of CSPs with different chiral selectors had been prepared. Silica gel immobilized CSP is especially attached great attention because of its high solvent tolerance and stability. In this paper, the new type of CSPs prepared by using chiral single molecules, polysaccharides, cyclodextrins, macrocyclic antibiotics, crown ethers, calixarenes and alkaloids as chiral selectors are summarized, and the development prospect of immobilized CSPs are also discussed.
手性分离在生物医药及其他领域中至关重要。高效液相色谱法（HPLC）因其经济、快速且高效的特点而被广泛应用于手性分离与分析。手性固定相（CSP）是在HPLC中实现手性拆分的关键。同时，制备有效CSP的关键在于手性选择剂的筛选。近年来，已制备出许多带有不同手性选择剂的CSP。硅胶固定化CSP因其高耐溶剂性和稳定性而备受关注。本文综述了以手性单分子、多糖、环糊精、大环抗生素、冠醚、杯芳烃和生物碱作为手性选择剂制备的新型CSP，并讨论了固定化CSP的发展前景。
