破坏细胞骨架的药物可增强生长因子和激素对DNA合成起始的作用。
Cytoskeleton-disrupting drugs enhance effect of growth factors and hormones on initiation of DNA synthesis.
作者信息
Otto A M, Zumbé A, Gibson L, Kubler A M, Jimenez de Asua L
出版信息
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6435-8. doi: 10.1073/pnas.76.12.6435.
Abstract
Addition of growth factors, such as prostaglandin F2 alpha or fibroblastic growth factor, to quiescent Swiss mouse 3T3 cells resulted in an abrupt increase in the rate of initiation of DNA synthesis after a lag phase of 13-15 hr. This increase could be quantified by a rate constant k. Addition of colchicine, Colcemid, or vinblastine had a synergistic effect on the initiation of DNA synthesis triggered by PGF2 alpha or FGF by increasing the value of k. These drugs alone had no effect. Colchicine had a synergistic effect only if added within 8 hr of the PGF2 alpha or FGF addition. Also, colchicine exerted its full effect when it was present only for the first 5 hr with either growth factor. These results suggest that an intact cytoskeleton is not required for the initiation of DNA synthesis. Furthermore, cytoskeleton-disrupting drugs enhance the stimulatory effect of the growth factors.
摘要
向静止的瑞士小鼠3T3细胞中添加生长因子，如前列腺素F2α或成纤维细胞生长因子，在13 - 15小时的延迟期后，DNA合成起始速率会突然增加。这种增加可以用速率常数k来量化。添加秋水仙碱、秋水仙酰胺或长春花碱，通过增加k值，对由PGF2α或FGF触发的DNA合成起始具有协同作用。这些药物单独使用没有效果。秋水仙碱只有在添加PGF2α或FGF后8小时内添加才有协同作用。此外，秋水仙碱仅在与任一生长因子共同存在的前5小时发挥其全部作用。这些结果表明，DNA合成起始不需要完整的细胞骨架。此外，破坏细胞骨架的药物可增强生长因子的刺激作用。
相似文献
1
Cytoskeleton-disrupting drugs enhance effect of growth factors and hormones on initiation of DNA synthesis.破坏细胞骨架的药物可增强生长因子和激素对DNA合成起始的作用。
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6435-8. doi: 10.1073/pnas.76.12.6435.
2
Microtubule-disrupting agents affect two different events regulating the initiation of DNA synthesis in Swiss 3T3 cells.微管破坏剂影响瑞士3T3细胞中调控DNA合成起始的两个不同事件。
Proc Natl Acad Sci U S A. 1981 May;78(5):3063-7. doi: 10.1073/pnas.78.5.3063.
3
Antitubulin agents enhance the stimulation of DNA synthesis by polypeptide growth factors in 3T3 mouse fibroblasts.抗微管蛋白药物可增强多肽生长因子对3T3小鼠成纤维细胞中DNA合成的刺激作用。
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3909-12. doi: 10.1073/pnas.76.8.3909.
4
The stimulation of the initiation of DNA synthesis by fibroblast growth factor in Swiss 3T3 cells: interactions with hormones during the pre-replicative phase.成纤维细胞生长因子对瑞士3T3细胞中DNA合成起始的刺激作用：复制前期与激素的相互作用
J Cell Physiol. 1980 Apr;103(1):77-85. doi: 10.1002/jcp.1041030112.
5
Two growth factors and two hormones regulate initiation of DNA synthesis in cultured mouse cells through different pathways of events.两种生长因子和两种激素通过不同的事件途径调节培养的小鼠细胞中DNA合成的起始。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):1004-8. doi: 10.1073/pnas.78.2.1004.
6
Colchicine enhances the effect of growth factors on the initiation of DNA synthesis in sparse and confluent swiss 3T3 cells.秋水仙碱增强生长因子对稀疏和汇合的瑞士3T3细胞中DNA合成起始的作用。
Cell Biol Int Rep. 1981 Jul;5(7):717-24. doi: 10.1016/0309-1651(81)90193-4.
7
Epidermal growth factor initiates DNA synthesis after a time-dependent sequence of regulatory events in Swiss 3T3 cells--interactions with hormones and growth factors.表皮生长因子在瑞士3T3细胞中一系列时间依赖性的调节事件后启动DNA合成——与激素和生长因子的相互作用。
J Cell Physiol. 1981 Aug;108(2):145-53. doi: 10.1002/jcp.1041080205.
8
Initiation of DNA synthesis by microtubule disruption in quiescent rat 3Y1 cells.通过破坏静止大鼠3Y1细胞中的微管来启动DNA合成。
Eur J Biochem. 1989 Aug 1;183(2):275-80. doi: 10.1111/j.1432-1033.1989.tb14924.x.
9
Colchicine acts as a progression factor to initiate DNA synthesis in quiescent Balb/c 3T3 cells.秋水仙碱作为一种促进因子，可启动静止的Balb/c 3T3细胞中的DNA合成。
FEBS Lett. 1988 Aug 15;236(1):19-22. doi: 10.1016/0014-5793(88)80277-1.
10
Microtubule-disrupting agents can independently affect the prereplicative period and the entry into S phase stimulated by prostaglandin F2 alpha and fibroblastic growth factor.破坏微管的药物能够独立影响复制前期以及由前列腺素F2α和成纤维细胞生长因子刺激而进入S期的过程。
J Cell Physiol. 1983 Apr;115(1):15-22. doi: 10.1002/jcp.1041150104.
引用本文的文献
1
Distinct Relations of Microtubules and Actin Filaments with Dendritic Architecture.微管和肌动蛋白丝与树突结构的独特关系。
iScience. 2020 Nov 27;23(12):101865. doi: 10.1016/j.isci.2020.101865. eCollection 2020 Dec 18.
2
The cytoskeletal network controls c-Jun expression and glucocorticoid receptor transcriptional activity in an antagonistic and cell-type-specific manner.细胞骨架网络以一种拮抗且细胞类型特异性的方式控制c-Jun表达和糖皮质激素受体转录活性。
Mol Cell Biol. 1999 Mar;19(3):1742-50. doi: 10.1128/MCB.19.3.1742.
3
Cytoskeleton-dependent activation of the inducible nitric oxide synthase in cultured aortic smooth muscle cells.培养的主动脉平滑肌细胞中细胞骨架依赖性诱导型一氧化氮合酶的激活
Br J Pharmacol. 1996 Jul;118(5):1085-94. doi: 10.1111/j.1476-5381.1996.tb15510.x.
4
Emerging drug treatments for solid tumours.实体瘤的新兴药物治疗方法。
Drugs. 1996 Jan;51(1):45-72. doi: 10.2165/00003495-199651010-00005.
5
Cytoskeleton modifications induced by phenobarbital, 2-acetylaminofluorene and 4-acetylaminofluorene in normal and initiated/selected hepatocytes: relation with the "resistant" phenotype.苯巴比妥、2-乙酰氨基芴和4-乙酰氨基芴在正常及起始/选择的肝细胞中诱导的细胞骨架修饰：与“抗性”表型的关系
Cell Biol Toxicol. 1993 Jan-Mar;9(1):61-76. doi: 10.1007/BF00755140.
6
7
Taxol inhibits neointimal smooth muscle cell accumulation after angioplasty in the rat.紫杉醇可抑制大鼠血管成形术后新生内膜平滑肌细胞的积聚。
J Clin Invest. 1995 Apr;95(4):1869-76. doi: 10.1172/JCI117867.
8
Dissociation of tumour-promoter-induced effects on prostaglandin release, polyamine synthesis and cell proliferation of 3T3 cells.肿瘤启动子对3T3细胞前列腺素释放、多胺合成和细胞增殖诱导作用的解离。
Biochem J. 1981 Mar 15;194(3):975-82. doi: 10.1042/bj1940975.
9
Microtubule-disrupting agents affect two different events regulating the initiation of DNA synthesis in Swiss 3T3 cells.微管破坏剂影响瑞士3T3细胞中调控DNA合成起始的两个不同事件。
Proc Natl Acad Sci U S A. 1981 May;78(5):3063-7. doi: 10.1073/pnas.78.5.3063.
10
Two growth factors and two hormones regulate initiation of DNA synthesis in cultured mouse cells through different pathways of events.两种生长因子和两种激素通过不同的事件途径调节培养的小鼠细胞中DNA合成的起始。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):1004-8. doi: 10.1073/pnas.78.2.1004.
本文引用的文献
1
Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.对培养的小鼠胚胎细胞生长及其发育成既定细胞系的定量研究。
J Cell Biol. 1963 May;17(2):299-313. doi: 10.1083/jcb.17.2.299.
2
Biochemical events in the animal cell cycle.动物细胞周期中的生化事件。
Fed Proc. 1969 Nov-Dec;28(6):1780-9.
3
Control of the initiation of DNA synthesis in 3T3 cells: low-molecular weight nutrients.3T3细胞中DNA合成起始的调控：低分子量营养素
Proc Natl Acad Sci U S A. 1974 Aug;71(8):2942-5. doi: 10.1073/pnas.71.8.2942.
4
Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth.一种成纤维细胞生长因子的定位及其单独作用以及与氢化可的松共同作用对3T3细胞生长的影响。
Nature. 1974 May 10;249(453):123-7. doi: 10.1038/249123a0.
5
Microtubules as drug receptors: pharmacological properties of microtubule protein.作为药物受体的微管：微管蛋白的药理学特性
Ann N Y Acad Sci. 1975 Jun 30;253:213-31. doi: 10.1111/j.1749-6632.1975.tb19201.x.
6
Stimulation of DNA synthesis and cell division in a chemically defined medium: effect of epidermal growth factor, insulin and vitamin B12 on resting cultures of 3T6 cells.在化学成分确定的培养基中刺激DNA合成和细胞分裂：表皮生长因子、胰岛素和维生素B12对3T6细胞静止培养物的影响。
Biochem Biophys Res Commun. 1976 Nov 22;73(2):271-8. doi: 10.1016/0006-291x(76)90703-8.
7
Continuous protein synthesis is required to maintain the probability of entry into S phase.需要持续的蛋白质合成来维持进入S期的可能性。
Cell. 1977 Sep;12(1):311-7. doi: 10.1016/0092-8674(77)90209-4.
8
Cells regulate their proliferation through alterations in transition probability.细胞通过改变转变概率来调节其增殖。
J Cell Physiol. 1977 Jun;91(3):345-55. doi: 10.1002/jcp.1040910304.
9
Animal cell cycle.动物细胞周期。
Annu Rev Biochem. 1978;47:715-50. doi: 10.1146/annurev.bi.47.070178.003435.
10
Direct visualization of binding, aggregation, and internalization of insulin and epidermal growth factor on living fibroblastic cells.胰岛素和表皮生长因子在活成纤维细胞上的结合、聚集及内化的直接可视化。
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2659-63. doi: 10.1073/pnas.75.6.2659.
Zotero 插件