Otto A M, Zumbé A, Gibson L, Kubler A M, Jimenez de Asua L
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6435-8. doi: 10.1073/pnas.76.12.6435.
Addition of growth factors, such as prostaglandin F2 alpha or fibroblastic growth factor, to quiescent Swiss mouse 3T3 cells resulted in an abrupt increase in the rate of initiation of DNA synthesis after a lag phase of 13-15 hr. This increase could be quantified by a rate constant k. Addition of colchicine, Colcemid, or vinblastine had a synergistic effect on the initiation of DNA synthesis triggered by PGF2 alpha or FGF by increasing the value of k. These drugs alone had no effect. Colchicine had a synergistic effect only if added within 8 hr of the PGF2 alpha or FGF addition. Also, colchicine exerted its full effect when it was present only for the first 5 hr with either growth factor. These results suggest that an intact cytoskeleton is not required for the initiation of DNA synthesis. Furthermore, cytoskeleton-disrupting drugs enhance the stimulatory effect of the growth factors.
向静止的瑞士小鼠3T3细胞中添加生长因子,如前列腺素F2α或成纤维细胞生长因子,在13 - 15小时的延迟期后,DNA合成起始速率会突然增加。这种增加可以用速率常数k来量化。添加秋水仙碱、秋水仙酰胺或长春花碱,通过增加k值,对由PGF2α或FGF触发的DNA合成起始具有协同作用。这些药物单独使用没有效果。秋水仙碱只有在添加PGF2α或FGF后8小时内添加才有协同作用。此外,秋水仙碱仅在与任一生长因子共同存在的前5小时发挥其全部作用。这些结果表明,DNA合成起始不需要完整的细胞骨架。此外,破坏细胞骨架的药物可增强生长因子的刺激作用。