补体因子B的Bb片段的氨基酸序列。主要溴化氰裂解肽（CB-II）的序列以及Bb片段序列的完成。
Amino acid sequence of the Bb fragment from complement Factor B. Sequence of the major cyanogen bromide-cleavage peptide (CB-II) and completion of the sequence of the Bb fragment.
作者信息
Christie D L, Gagnon J
出版信息
Biochem J. 1983 Jan 1;209(1):61-70. doi: 10.1042/bj2090061.
Abstract
The amino acid sequence of peptide CB-II, the major product (mol.wt. 30 000) of CNBr cleavage of fragment Bb from human complement Factor B, is given. The sequence was obtained from peptides derived by trypsin cleavage of peptide CB-II and clostripain digestion of fragment Bb. Cleavage of two Asn-Gly bonds in peptide CB-II was also found useful. These results, along with those presented in the preceding paper [Gagnon & Christie (1983) Biochem. J. 209, 51-60], yield the complete sequence of the 505 amino acid residues of fragment Bb. The C-terminal half of the molecule shows strong homology of sequence with serine proteinases. Factor B has a catalytic chain (fragment Bb) with a molecular weight twice that of proteinases previously described, suggesting that it is a novel type of serine proteinase, probably with a different activation mechanism.
摘要
给出了人补体因子B的Bb片段经溴化氰裂解后的主要产物（分子量30000）肽CB-II的氨基酸序列。该序列是从经胰蛋白酶裂解肽CB-II和经梭菌蛋白酶消化Bb片段得到的肽段中获得的。还发现裂解肽CB-II中的两个天冬酰胺-甘氨酸键很有用。这些结果与前文[加尼翁和克里斯蒂（1983年）《生物化学杂志》209卷，51 - 60页]所呈现的结果一起，得出了Bb片段505个氨基酸残基的完整序列。该分子的C端一半显示出与丝氨酸蛋白酶有很强的序列同源性。因子B有一条催化链（Bb片段），其分子量是先前描述的蛋白酶的两倍，这表明它是一种新型的丝氨酸蛋白酶，可能具有不同的激活机制。
相似文献
1
Amino acid sequence of the Bb fragment from complement Factor B. Sequence of the major cyanogen bromide-cleavage peptide (CB-II) and completion of the sequence of the Bb fragment.补体因子B的Bb片段的氨基酸序列。主要溴化氰裂解肽（CB-II）的序列以及Bb片段序列的完成。
Biochem J. 1983 Jan 1;209(1):61-70. doi: 10.1042/bj2090061.
2
Amino acid sequence of the Bb fragment from human complement Factor B. Alignment of the cyanogen bromide-cleavage peptides.人补体因子B的Bb片段的氨基酸序列。溴化氰裂解肽的比对。
Biochem J. 1983 Jan 1;209(1):51-60. doi: 10.1042/bj2090051.
3
The complete amino acid sequence of chicken skeletal-muscle enolase.鸡骨骼肌烯醇化酶的完整氨基酸序列。
Biochem J. 1986 May 15;236(1):115-26. doi: 10.1042/bj2360115.
4
Isolation, characterization and N-terminal sequences of the CNBr-cleavage peptides from human complement Factor B. Localization of a free thiol group and a sequence defining the site cleaved by factor D.人补体因子B中溴化氰裂解肽段的分离、特性鉴定及N端序列。游离巯基的定位以及定义被因子D裂解位点的序列。
Biochem J. 1982 Mar 1;201(3):555-67. doi: 10.1042/bj2010555.
5
Partial sequence of human complement component factor B: novel type of serine protease.人类补体成分B因子的部分序列：新型丝氨酸蛋白酶
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4923-7. doi: 10.1073/pnas.77.8.4923.
6
CNBr cleavage of the light chain of human complement factor I and alignment of the fragments.人补体因子I轻链的溴化氰裂解及片段比对
Biochem J. 1986 Jan 15;233(2):339-45. doi: 10.1042/bj2330339.
7
Structure and activation of complement components C2 and factor B.补体成分C2和B因子的结构与激活
Philos Trans R Soc Lond B Biol Sci. 1984 Sep 6;306(1129):301-9. doi: 10.1098/rstb.1984.0091.
8
Characterization of the CNBr peptides generated from the factor B cleavage fragments, Ba and Bb, by molecular exclusion high performance liquid chromatography.
Immunol Commun. 1982;11(1):47-58. doi: 10.3109/08820138209050723.
9
The catalytic chain of human complement subcomponent C1r. Purification and N-terminal amino acid sequences of the major cyanogen bromide-cleavage fragments.人补体亚成分C1r的催化链。主要溴化氰裂解片段的纯化及N端氨基酸序列
Biochem J. 1982 Jan 1;201(1):49-59. doi: 10.1042/bj2010049.
10
Isolation and characterization of the cyanogen bromide peptides from the B fragment of diphtheria toxin.
Biochim Biophys Acta. 1978 Jul 21;535(1):54-65. doi: 10.1016/0005-2795(78)90032-6.
引用本文的文献
1
Production and functional activity of a recombinant von Willebrand factor-A domain from human complement factor B.人补体因子B重组血管性血友病因子A结构域的产生及功能活性
Biochem J. 1999 Sep 15;342 Pt 3(Pt 3):625-32.
2
Amino acid sequence of the Bb fragment from human complement Factor B. Alignment of the cyanogen bromide-cleavage peptides.人补体因子B的Bb片段的氨基酸序列。溴化氰裂解肽的比对。
Biochem J. 1983 Jan 1;209(1):51-60. doi: 10.1042/bj2090051.
3
Internal homologies of the Ba fragment from human complement component Factor B, a class III MHC antigen.人类补体成分B因子（一种III类主要组织相容性复合体抗原）的Ba片段的内部同源性。
EMBO J. 1984 Jan;3(1):153-7. doi: 10.1002/j.1460-2075.1984.tb01776.x.
4
Molecular cloning and characterization of the gene coding for human complement protein factor B.编码人补体蛋白B因子的基因的分子克隆与特性分析
Proc Natl Acad Sci U S A. 1983 Jul;80(14):4464-8. doi: 10.1073/pnas.80.14.4464.
5
Isolation of cDNA clones for human complement component C2.人补体成分C2的cDNA克隆的分离
Proc Natl Acad Sci U S A. 1984 Feb;81(4):1212-5. doi: 10.1073/pnas.81.4.1212.
6
A factor IX mutation, verified by direct genomic sequencing, causes haemophilia B by a novel mechanism.经直接基因组测序验证的一种因子IX突变通过一种新机制导致B型血友病。
EMBO J. 1988 Oct;7(10):3009-15. doi: 10.1002/j.1460-2075.1988.tb03164.x.
7
Primary structure of human complement component C2. Homology to two unrelated protein families.人补体成分C2的一级结构。与两个不相关蛋白质家族的同源性。
Biochem J. 1986 Oct 15;239(2):339-45. doi: 10.1042/bj2390339.
8
CNBr cleavage of the light chain of human complement factor I and alignment of the fragments.人补体因子I轻链的溴化氰裂解及片段比对
Biochem J. 1986 Jan 15;233(2):339-45. doi: 10.1042/bj2330339.
本文引用的文献
1
Isolation, characterization and N-terminal sequences of the CNBr-cleavage peptides from human complement Factor B. Localization of a free thiol group and a sequence defining the site cleaved by factor D.人补体因子B中溴化氰裂解肽段的分离、特性鉴定及N端序列。游离巯基的定位以及定义被因子D裂解位点的序列。
Biochem J. 1982 Mar 1;201(3):555-67. doi: 10.1042/bj2010555.
2
Structural evidence that complement factor B constitutes a novel class of serine protease.补体因子B构成一类新型丝氨酸蛋白酶的结构证据。
J Biol Chem. 1980 Sep 25;255(18):8472-6.
3
Factor D of the alternative pathway of human complement. Purification, alignment and N-terminal amino acid sequences of the major cyanogen bromide fragments, and localization of the serine residue at the active site.人补体替代途径的D因子。主要溴化氰片段的纯化、比对及N端氨基酸序列，以及活性位点丝氨酸残基的定位
Biochem J. 1980 Jun 1;187(3):863-74. doi: 10.1042/bj1870863.
4
Partial sequence of human complement component factor B: novel type of serine protease.人类补体成分B因子的部分序列：新型丝氨酸蛋白酶
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4923-7. doi: 10.1073/pnas.77.8.4923.
5
Amino acid sequence of the Bb fragment from human complement Factor B. Alignment of the cyanogen bromide-cleavage peptides.人补体因子B的Bb片段的氨基酸序列。溴化氰裂解肽的比对。
Biochem J. 1983 Jan 1;209(1):51-60. doi: 10.1042/bj2090051.
6
The catalytic chain of human complement subcomponent C1r. Purification and N-terminal amino acid sequences of the major cyanogen bromide-cleavage fragments.人补体亚成分C1r的催化链。主要溴化氰裂解片段的纯化及N端氨基酸序列
Biochem J. 1982 Jan 1;201(1):49-59. doi: 10.1042/bj2010049.
7
Clr and Cls subcomponents of human complement: two serine proteinases lacking the 'histidine-loop' disulphide bridge.人类补体的Clr和Cls亚成分：两种缺乏“组氨酸环”二硫键的丝氨酸蛋白酶。
Biosci Rep. 1981 Oct;1(10):779-84. doi: 10.1007/BF01114800.
8
Supports for reverse-phase high-performance liquid chromatography of large proteins.用于大蛋白质反相高效液相色谱的载体。
Anal Biochem. 1980 May 1;104(1):153-9. doi: 10.1016/0003-2697(80)90291-2.
9
Substrate binding site in bovine chymotrypsin A-gamma. A crystallographic study using peptide chloromethyl ketones as site-specific inhibitors.牛胰凝乳蛋白酶A-γ中的底物结合位点。使用肽氯甲基酮作为位点特异性抑制剂的晶体学研究。
Biochemistry. 1971 Sep 28;10(20):3728-38. doi: 10.1021/bi00796a014.
10
The crystal and molecular structure of DIP-inhibited bovine trypsin at2.7Angstrom resolution.
Cold Spring Harb Symp Quant Biol. 1972;36:125-40. doi: 10.1101/sqb.1972.036.01.018.
Zotero 插件