Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
State Key Laboratory of Safety Evaluation for New Drugs, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, China.
Sci Rep. 2018 Jan 26;8(1):1698. doi: 10.1038/s41598-018-20083-z.
Quzhou Fructus Aurantii (QFA) is an authentic herb of local varieties in Zhejiang, China, which is usually used to treat gastrointestinal illnesses, but its effects on respiratory inflammation have not been reported yet. In our study, the anti-inflammatory activity of QFA extract (QFAE) was evaluated on copper sulfate pentahydrate (CuSO·5HO)-induced transgenic neutrophil fluorescent zebrafish model. QFAE showed a significant effect of anti-inflammation in CuSO·5HO-induced zebrafish by reducing the neutrophil number in the inflammatory site. We investigated the anti-inflammatory activity of QFAE on lipopolysaccharide (LPS)-induced acute lung injury (ALI) mice models and RAW 264.7 cells. QFAE had an anti-inflammatory effect on reducing total cells, neutrophils, and macrophages in BALF and attenuated alveolus collapse, neutrophils infiltration, lung W/D ratio, myeloperoxidase (MPO) protein expression and other pulmonary histological changes in lung tissues, as well as hematological changes. Levels of pro-inflammatory cytokines, including TNF, IL-6, IFN-γ, MCP-1, and IL-12p70, were decreased, whereas anti-inflammatory cytokine IL-10 was increased after treatment with QFAE both in vivo and in vitro. In summary, our results suggested that QFAE had apparent anti-inflammatory effects on CuSO·5HO-induced zebrafish, LPS-induced ALI mice, and RAW 264.7 cells. Furthermore, QFAE may be a therapeutic drug to treat ALI/ARDS and other respiratory inflammations.
衢州枳实（QFA）是中国浙江的一种地道地方品种草药，通常用于治疗胃肠道疾病，但尚未报道其对呼吸道炎症的作用。在我们的研究中，评估了五水硫酸铜（CuSO·5HO）诱导的转基因荧光斑马鱼模型中枳实提取物（QFAE）的抗炎活性。QFAE 通过减少炎症部位的中性粒细胞数量，在 CuSO·5HO 诱导的斑马鱼中表现出显著的抗炎作用。我们研究了 QFAE 对脂多糖（LPS）诱导的急性肺损伤（ALI）小鼠模型和 RAW 264.7 细胞的抗炎活性。QFAE 具有抗炎作用，可减少 BALF 中的总细胞、中性粒细胞和巨噬细胞，并减轻肺泡塌陷、中性粒细胞浸润、肺 W/D 比、髓过氧化物酶（MPO）蛋白表达和肺组织中的其他肺组织学变化，以及血液学变化。促炎细胞因子，包括 TNF、IL-6、IFN-γ、MCP-1 和 IL-12p70 的水平降低，而 QFAE 处理后体内和体外抗炎细胞因子 IL-10 的水平升高。综上所述，我们的结果表明，QFAE 对 CuSO·5HO 诱导的斑马鱼、LPS 诱导的 ALI 小鼠和 RAW 264.7 细胞具有明显的抗炎作用。此外，QFAE 可能是治疗 ALI/ARDS 和其他呼吸道炎症的治疗药物。
