Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Turkey.
Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Turkey.
Food Chem Toxicol. 2018 Mar;113:90-98. doi: 10.1016/j.fct.2018.01.034. Epub 2018 Jan 31.
Pendimethalin and trifluralin are widely used dinitroaniline herbicides. Both compounds can be found as residue levels in agricultural products. This study was conducted in order to provide necessary information for the risk assessment of pendimethalin and trifluralin. In this study, reactive oxygen species (ROS) levels were measured to examine the potential of both compounds to induce oxidative damage in Chinese hamster lung fibroblast (V79) cells. Also, the genotoxic effects of pendimethalin and trifluralin at the concentration range of 1-500 μM was determined. Single cell gel electrophoresis (comet) and micronucleus assays were used on human peripheral lymphocytes and V79 cells for the genotoxicity assessment. The cell viability of two dinitroaniline herbicides were determined by the use of neutral red uptake assay on V79 cells. IC values were determined as 66 μM and 128 μM for pendimethalin and trifluralin, respectively. They significantly increased ROS levels on V79 cells for 1-24 h. Both herbicides significantly induced the DNA damage and showed genotoxicity on lymphocytes and V79 cells. Micronucleus frequency increased significantly after pendimethalin and trifluralin treatment of the lymphocytes and V79 cells. Therefore, we concluded that both of the herbicides induced the genotoxicity through the activation of oxidative stress pathway and chromosomal damage.
二甲戊灵和氟乐灵是广泛使用的二硝基苯胺类除草剂。这两种化合物都可以在农产品中残留。本研究旨在为评估二甲戊灵和氟乐灵的风险提供必要的信息。在这项研究中，测量了活性氧（ROS）水平，以检查这两种化合物在中华仓鼠肺成纤维细胞（V79）中诱导氧化损伤的潜力。此外，还测定了二甲戊灵和氟乐灵在 1-500 μM 浓度范围内的遗传毒性。单细胞凝胶电泳（彗星）和微核试验分别用于人外周血淋巴细胞和 V79 细胞的遗传毒性评估。使用 V79 细胞中的中性红摄取试验测定了两种二硝基苯胺类除草剂的细胞活力。二甲戊灵和氟乐灵的 IC 值分别为 66 μM 和 128 μM。它们在 V79 细胞上显著增加了 1-24 小时的 ROS 水平。这两种除草剂均显著诱导了淋巴细胞和 V79 细胞的 DNA 损伤，并表现出遗传毒性。在用二甲戊灵和氟乐灵处理淋巴细胞和 V79 细胞后，微核频率显著增加。因此，我们得出结论，这两种除草剂通过激活氧化应激途径和染色体损伤诱导遗传毒性。
