Department of Plant Production, College of Agricultural and Technological Sciences, São Paulo State University (UNESP), Dracena, SP, 17900-000, Brazil.
Sci Rep. 2022 Jun 2;12(1):9225. doi: 10.1038/s41598-022-13147-8.
This study aimed to propose an eco-compatible strategy to mitigate the possible environmental contamination caused by tebuthiuron. Therefore, we screened potential tebuthiuron-degrading microorganisms from conventional (CS) and no-till (NTS) systems producing sugarcane. Then, they were bioprospected for their ability of decomposing the target-molecule at 2.48 mmol g and 4.96 mmol g into CO via respirometry. Integrating microbiota from CS and NTS into an advantageously synergistic bacterial pool produced the highest specific-growth rate of CO of 89.60 mg day, so outstripped the other inoculum. The bacterial CN-NTS framework notably stabilized the sigmoidal Gompertz curve on microbial degradation earliest and enabled the seeds of Lactuca sativa to germinate healthiest throughout ecotoxicological bioassay for cross-validation. Our study is preliminary, but timely to provide knowledge of particular relevance to progress in the field's prominence in remediating terrestrial ecosystems where residual tebuthiuron can persist and contaminate. The analytical insights will act as an opening of solutions to develop high-throughput biotechnological strategies for environmental decontamination.
本研究旨在提出一种生态兼容的策略，以减轻特丁津可能造成的环境污染。因此，我们从生产甘蔗的常规耕作（CS）和免耕（NTS）系统中筛选出具有特丁津降解能力的潜在微生物。然后，通过呼吸计法测定它们在 2.48mmol g 和 4.96mmol g 下将目标分子分解为 CO 的能力。将 CS 和 NTS 的微生物群落整合到一个有利的协同细菌池中，产生了最高的 CO 比生长速率为 89.60mg·day，因此超过了其他接种物。细菌 CN-NTS 框架显著稳定了微生物降解最早的 sigmoidal Gompertz 曲线，并使生菜种子在生态毒理学生物测定中最健康地发芽，从而进行交叉验证。我们的研究是初步的，但及时提供了特别相关的知识，以促进该领域在修复特丁津残留并污染的陆地生态系统方面的突出地位。分析见解将成为开发用于环境净化的高通量生物技术策略的解决方案的起点。
