Han Z G, Zhang Y L, Wu H, Cheng W B, Liang C Y, Zhong F, Gao K, Xu H F
Department of Operational Control, Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2018 Jan 10;39(1):67-71. doi: 10.3760/cma.j.issn.0254-6450.2018.01.014.
To understand the characteristics and dynamic of HIV-1 subtype distribution in men who have sex with men (MSM) in Guangzhou between 2008 and 2015. HIV-1 RNAs were extracted from serum samples of the individuals newly diagnosed with HIV-1 infection among MSM living in Guangzhou between 2008 and 2015. The gene segments of HIV-1 genome from these RNA samples were amplified by nested reverse transcription polymerase chain reaction (nested-PCR) and were sequenced. Subsequently, the phylogenetic tree was reconstructed using sequences of samples and references together and the subtype of HIV-1 was determined. The distributions of HIV-1 subtypes detected in MSM with different demographic characteristics in different years were compared. A total of 2 210 pol gene segments were successfully obtained from 2 473 serum samples of the MSM. The average age of 2 210 MSM was 30.19 years with standard deviation of 8.22 years, the unmarried MSM and those in Han ethnic group accounted for 73.39% and 90.81%, respectively. The proportion of subtype CRF07_BC (38.10%) was highest, followed by CRF01_AE (34.84%), CRF55_01B (14.62%), B (6.06%), URFs (3.58%), CRF59_01B (2.17%) and other subtypes (0.63%). The annual proportions of subtype B (=0.000, 99%:0.000-0.000), CRF07_BC ((2)=14.965, =0.036), CRF55_01B ((2)=18.161, =0.011) and URFs (=0.001, 99% : 0.000-0.001) were significantly different. The proportion of subtype B showed a gradual decrease from 14.08% to 4.33% (=0.000, 99%: 0.000-0.000), while the proportion of URFs rapidly increased from 0% to 6.40% (=0.000, 99% : 0.000-0.000). The rate of URFs was significantly higher in farmers and migrant workers than in other groups (=0.017, 99%: 0.014- 0.020) and the rate of URFs was higher in individuals who had multi sexual partners ((2)=5.733, =0.017). CRF07_BC and CRF01_AE were the predominant HIV-1 subtypes and multiple subtypes co-circulated among MSM in Guangzhou between 2008 and 2015. The recombinations of HIV-1 continue to occur in MSM. Strengthening behavioral intervention for farmers, migrant workers and individuals who have multi sexual partners has the important epidemiological significance against the emerging and circulating of the novel recombinant virus among MSM in Guangzhou.
了解2008年至2015年广州男男性行为者（MSM）中HIV-1亚型分布的特征及动态变化。从2008年至2015年居住在广州的MSM中，新诊断为HIV-1感染个体的血清样本中提取HIV-1 RNA。通过巢式逆转录聚合酶链反应（巢式PCR）扩增这些RNA样本中HIV-1基因组的基因片段并进行测序。随后，将样本和参考序列一起用于重建系统发育树，并确定HIV-1的亚型。比较不同年份不同人口学特征的MSM中检测到的HIV-1亚型分布情况。从2473例MSM的血清样本中成功获得了2210个pol基因片段。2210例MSM的平均年龄为30.19岁，标准差为8.22岁，未婚MSM和汉族MSM分别占73.39%和90.81%。CRF07_BC亚型比例最高（38.10%），其次是CRF01_AE（34.84%）、CRF55_01B（14.62%）、B（6.06%）、URFs（3.58%）、CRF59_01B（2.17%）和其他亚型（0.63%）。B亚型（=0.000，99%：0.000 - 0.000）、CRF07_BC（（2）=14.965，=0.036）、CRF55_01B（（2）=18.161，=0.011）和URFs（=0.001，99%：0.000 - 0.001）的年度比例有显著差异。B亚型比例从14.08%逐渐下降至4.33%（=0.000，99%：0.000 - 0.000），而URFs比例从0%迅速上升至6.40%（=0.000，99%：0.000 - 0.000）。农民和农民工中URFs比例显著高于其他群体（=0.017，99%：0.014 - 0.020），多性伴个体中URFs比例更高（（2）=5.733，=0.017）。CRF07_BC和CRF01_AE是2008年至2015年广州MSM中主要的HIV-1亚型，多种亚型共同流行。HIV-1重组在MSM中持续发生。加强对农民、农民工和多性伴个体的行为干预，对于预防新型重组病毒在广州MSM中的出现和传播具有重要的流行病学意义。
