Blanken R, van der Valk P G, Nater J P
Derm Beruf Umwelt. 1986 Jan-Feb;34(1):5-9.
The value of laser-Doppler flowmetry was assessed in the evaluation of increased microvascular blood flow caused by the application of low concentrations of irritant compounds on the skin. Laser-Doppler flow values were compared with a visual scoring system for erythema and with skin vapour loss measurements. To this end patch tests were performed on the volar side of the forearm of 24 healthy Caucasian volunteers. The test substances were sodium lauryl sulphate 0.5 g/v%, 1 g/v%, 2 g/v% and phenol 5.0 g/v% in aqua dest. Distilled water served as a control. Laser-Doppler flow and skin vapour loss measurements were performed before and after an application time of 24 h. Data obtained with skin vapour loss measurements and with the visual scoring system demonstrated a statistically significant contribution of substance- as well as subject-related factors to the obtained scoring patterns. The results of laser-Doppler flow measurements were less consistent and could only partly be explained in this way, indicating that other factors also influence the microvascular blood flow in the skin. In this investigation laser-Doppler flowmetry and the visual scoring system were equally sensitive. The results of all three methods make it clear that (analogous to experience in daily life) barrier function impairment and inflammatory response caused by irritant compounds vary in different subjects. For the elucidation of the pathogenesis of irritant contact dermatitis further (longitudinal) investigations on the possible predictive value of skin blood flow measurements will be necessary.
在评估低浓度刺激性化合物作用于皮肤所引起的微血管血流增加时，对激光多普勒血流仪的价值进行了评估。将激光多普勒血流值与红斑的视觉评分系统以及皮肤蒸发性失水量测量结果进行了比较。为此，对24名健康的白种人志愿者前臂掌侧进行了斑贴试验。受试物质为0.5 g/v%、1 g/v%、2 g/v%的十二烷基硫酸钠以及5.0 g/v%的苯酚水溶液。蒸馏水作为对照。在24小时的敷用时间前后进行激光多普勒血流和皮肤蒸发性失水量测量。皮肤蒸发性失水量测量和视觉评分系统所获得的数据表明，物质相关因素和受试者相关因素对所获得的评分模式有统计学上的显著贡献。激光多普勒血流测量结果不太一致，只能部分地以此方式解释，这表明其他因素也会影响皮肤中的微血管血流。在本研究中，激光多普勒血流仪和视觉评分系统同样敏感。所有三种方法的结果都清楚表明（类似于日常生活中的经验），刺激性化合物引起的屏障功能损害和炎症反应在不同受试者中有所不同。为了阐明刺激性接触性皮炎的发病机制，有必要进一步（纵向）研究皮肤血流测量的可能预测价值。
