Liu Hai-Hui, Lu Chen-Na, Zhu Xuan-Xuan, Bai Lu, Lin Li-Mei, Chen Qian-Wen, Feng Wei-Hong, Liao Duan-Fang, Li Chun
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Qianjin Pharmaceutical Co., Ltd.,Zhuzhou 412007, China.
Zhongguo Zhong Yao Za Zhi. 2023 May;48(10):2781-2791. doi: 10.19540/j.cnki.cjcmm.20230322.201.
Rosae Radix et Rhizoma is a herbal medicine in a variety of famous Chinese patent medicines, while the quality standard for this medicine remains to be developed due to the insufficient research on the quality of Rosae Radix et Rhizoma from different sources. Therefore, this study comprehensively analyzed the components in Rosae Radix et Rhizoma of different sources from the aspects of extract, component category content, identification based on thin-lay chromatography, active component content determination, and fingerprint, so as to improve the quality control. The results showed that the content of chemical components varied in the samples of different sources, while there was little difference in the chemical composition among the samples. The content of components in the roots of Rosa laevigata was higher than that in the other two species, and the content of components in the roots was higher than that in the stems. The fingerprints of triterpenoids and non-triterpenoids were established, and the content of five main triterpenoids including multiflorin, rosamultin, myrianthic acid, rosolic acid, and tormentic acid in Rosae Radix et Rhizoma was determined. The results were consistent with those of major component categories. In conclusion, the quality of Rosae Radix et Rhizoma is associated with the plant species, producing area, and medicinal parts. The method established in this study lays a foundation for improving the quality standard of Rosae Radix et Rhizoma and provides data support for the rational use of the stem.
蔷薇根是多种著名中成药中的一味草药,然而由于对不同来源蔷薇根质量的研究不足,该药材的质量标准仍有待制定。因此,本研究从提取物、成分类别含量、薄层色谱鉴别、活性成分含量测定及指纹图谱等方面,对不同来源蔷薇根中的成分进行了综合分析,以完善质量控制。结果表明,不同来源样品中化学成分的含量存在差异,但化学成分组成差异不大。金樱子根中成分的含量高于其他两个品种,且根中成分的含量高于茎中。建立了三萜类和非三萜类的指纹图谱,并测定了蔷薇根中包括多花蔷薇苷、蔷薇红素、地榆酸、蔷薇酸和委陵菜酸在内的五种主要三萜类成分的含量。结果与主要成分类别一致。综上所述,蔷薇根的质量与植物品种、产地和药用部位有关。本研究建立的方法为提高蔷薇根的质量标准奠定了基础,并为合理利用其茎提供了数据支持。