金属化卟啉掺杂共轭聚合物纳米粒子用于脑和结直肠肿瘤细胞的高效光动力治疗。
Metallated porphyrin-doped conjugated polymer nanoparticles for efficient photodynamic therapy of brain and colorectal tumor cells.
机构信息
Universidad Nacional de Río Cuarto y CONICET, Instituto de Biotecnología Ambiental y Salud (INBIAS), Dto. Biología Molecular, Facultad de Ciencias Exactas Fisicoquímicas y Naturales, Río Cuarto (5800), Córdoba, Argentina.
Universidad Nacional de Río Cuarto y CONICET, Dto. Química, Facultad de Ciencias Exactas Fisicoquímicas y Naturales, Río Cuarto (5800), Córdoba, Argentina.
出版信息
Nanomedicine (Lond). 2018 Mar;13(6):605-624. doi: 10.2217/nnm-2017-0292. Epub 2018 Jan 29.
AIM
Assess biocompatibility, uptake and photodynamic therapy (PDT) mechanism of metallated porphyrin doped conjugated polymer nanoparticles (CPNs) in human brain and colorectal tumor cells and macrophages.
MATERIALS & METHODS: CPNs were developed employing 9,9-dioctylfluorene-alt-benzothiadiazole, an amphiphilic polymer (PS-PEG-COOH), and platinum octaethylporphyrin. T98G, SW480 and RAW 264.7 cell lines were exposed to CPNs to assess uptake and intracellular localization. Additionally, a PDT protocol using CPNs was employed for the in vitro killing of cancer and macrophage cell lines.
RESULTS & CONCLUSION: CPNs were well incorporated into glioblastoma and macrophage cells with localization in lysosomes. SW480 cells were less efficient incorporating CPNs with localization in the plasma membrane. In all cell lines PDT treatment was efficient inducing oxidative stress that triggered apoptosis.
目的
评估金属卟啉掺杂共轭聚合物纳米粒子（CPNs）在人脑和结直肠肿瘤细胞及巨噬细胞中的生物相容性、摄取和光动力疗法（PDT）机制。
材料与方法
采用 9,9-二辛基芴并[9,10-d]噻唑-2,7-二基-alt-苯并噻二唑（PS-PEG-COOH）等两亲性聚合物和铂八乙基卟啉制备 CPNs。将 T98G、SW480 和 RAW 264.7 细胞系暴露于 CPNs 中，以评估摄取和细胞内定位情况。此外，还采用 CPNs 的 PDT 方案对癌细胞和巨噬细胞系进行体外杀伤。
结果与结论
CPNs 很好地被整合到神经胶质瘤和巨噬细胞中，并定位于溶酶体中。SW480 细胞摄取 CPNs 的效率较低，CPNs 定位于细胞膜。在所有细胞系中，PDT 处理均能有效诱导氧化应激，从而触发细胞凋亡。
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