Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan
Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
Antimicrob Agents Chemother. 2018 Mar 27;62(4). doi: 10.1128/AAC.02035-17. Print 2018 Apr.
subsp. mainly causes disseminated infection in immunocompromised hosts, such as individuals with human immunodeficiency virus (HIV) infection, and pulmonary infection in immunocompetent hosts. However, many aspects of the different types of subsp. infection remain unclear. We examined the antibiotic susceptibilities and genotypes of subsp. isolates from different hosts by performing drug susceptibility testing using eight antibiotics (clarithromycin, rifampin, ethambutol, streptomycin, kanamycin, amikacin, ethionamide, and levofloxacin) and variable-number tandem-repeat (VNTR) typing analysis for 46 isolates from the sputa of HIV-negative patients with pulmonary subsp. disease without previous antibiotic treatment and 30 isolates from the blood of HIV-positive patients with disseminated subsp. disease. Interestingly, isolates from pulmonary subsp. disease patients were more resistant to seven of the eight drugs, with the exception being rifampin, than isolates from HIV-positive patients. Moreover, VNTR typing analysis showed that the strains examined in this study were roughly classified into three clusters, and the genetic distance from reference strain 104 for isolates from pulmonary subsp. disease patients was statistically significantly different from that for isolates from HIV-positive patients ( = 0.0018), suggesting that subsp. strains that cause pulmonary and disseminated disease have genetically distinct features. Significant differences in susceptibility to seven of the eight drugs, with the exception being ethambutol, were noted among the three clusters. Collectively, these results suggest that an association between the type of subsp. infection, drug susceptibility, and the VNTR genotype and the properties of subsp. strains associated with the development of pulmonary disease are involved in higher levels of antibiotic resistance.
该亚群主要引起免疫功能低下宿主（如人类免疫缺陷病毒 [HIV] 感染者）的全身性感染，以及免疫功能正常宿主的肺部感染。然而，该亚群的不同类型感染的许多方面仍不清楚。我们通过使用 8 种抗生素（克拉霉素、利福平、乙胺丁醇、链霉素、卡那霉素、阿米卡星、乙硫异烟胺和左氧氟沙星）进行药敏试验，并对 46 例未经抗生素治疗的 HIV 阴性肺部感染患者的痰液和 30 例 HIV 阳性播散性感染患者的血液中的 亚群 分离株进行可变数串联重复（VNTR）分型分析，研究了来自不同宿主的 亚群 分离株的抗生素敏感性和基因型。有趣的是，与 HIV 阳性播散性感染患者的分离株相比，来自肺部感染患者的分离株对除利福平以外的 8 种药物中的 7 种药物的耐药性更高。此外，VNTR 分型分析表明，本研究中检测到的菌株大致分为 3 个聚类，与肺部感染患者分离株的参考株 104 相比，HIV 阳性患者分离株的遗传距离具有统计学显著性差异（=0.0018），这表明引起肺部和播散性疾病的 亚群 菌株具有遗传上不同的特征。在 3 个聚类中，除乙胺丁醇外，对 8 种药物中的 7 种药物的敏感性存在显著差异。总的来说，这些结果表明， 亚群 感染类型、药敏性以及 VNTR 基因型与引起肺部疾病的 亚群 菌株特性之间存在关联，与更高水平的抗生素耐药性有关。
