Arlıer Sefa
South Florida University Faculty of Medicine, Department of Obstetrics and Gynecology, Tampa, USA.
University of Health Sciences, Adana Numune Training and Research Hospital, Clinic of Obstetrics and Gynecology, Adana, Turkey.
Turk J Obstet Gynecol. 2017 Dec;14(4):220-227. doi: 10.4274/tjod.78545. Epub 2017 Dec 30.
Increased leptin hormone and leptin receptor may enhance the generation of proinflammatory cytokines by endothelial cells and lead to endothelial dysfunction. This study assessed the umbilical cord endothelial leptin receptor levels in preeclampsia and investigated the effect of leptin on endothelial interleukin-8 (IL-8) production.
The association between IL-8 levels with leptin stimulation was investigated in leptin-treated human endothelial cells. Endothelial cell leptin receptor levels were evaluated using immunohistochemistry staining, and endothelial IL-8 protein expression by Western blot analysis. Data are presented as mean ± standard error of the mean (SEM). Statistical significance was analyzed using Student's t-test or Mann-Whitney U test and one-way analysis of variance.
Leptin receptor immunoreactivity increased significantly in umbilical cord venous and arterial endothelial cells in normal pregnancy (n=12) compared with preeclampsia (n=7) endothelial cells. The corresponding preeclampsia versus control histologic scores (mean ± SEM) were 67.9±8.8 vs. 127.6±23.1, (p=0.011) for the leptin receptor and 55.4±8,0 vs. 93.7±17.1 (p=0.035), respectively, for the vein endothelial cells. Leptin treatment significantly increased IL-8 protein levels (control vs. 100 and 1000 ng/mL, p=0.003).
The findings of increased umbilical cord endothelial leptin receptor levels in preeclampsia and increased endothelial IL-8 expression with exposure to higher leptin concentrations may indicate the contribution of leptin to endothelial dysfunction and increased neutrophil-endothelial interaction, which are significant pathophysiologic features of preeclampsia.
瘦素激素和瘦素受体增加可能会增强内皮细胞促炎细胞因子的生成，并导致内皮功能障碍。本研究评估了子痫前期患者脐带内皮瘦素受体水平，并研究了瘦素对内皮白细胞介素-8（IL-8）产生的影响。
在瘦素处理的人内皮细胞中研究了IL-8水平与瘦素刺激之间的关联。使用免疫组织化学染色评估内皮细胞瘦素受体水平，并通过蛋白质印迹分析评估内皮IL-8蛋白表达。数据以平均值±平均标准误差（SEM）表示。使用学生t检验或曼-惠特尼U检验以及单因素方差分析来分析统计学意义。
与子痫前期（n = 7）内皮细胞相比，正常妊娠（n = 12）脐带静脉和动脉内皮细胞中瘦素受体免疫反应性显著增加。子痫前期与对照的相应组织学评分（平均值±SEM），瘦素受体分别为67.9±8.8对127.6±23.1（p = 0.011），静脉内皮细胞分别为55.4±8.0对93.7±17.1（p = 0.035）。瘦素处理显著增加了IL-8蛋白水平（对照与100和1000 ng/mL相比，p = 0.003）。
子痫前期脐带内皮瘦素受体水平升高以及暴露于较高瘦素浓度时内皮IL-8表达增加的结果可能表明瘦素对内皮功能障碍和中性粒细胞-内皮细胞相互作用增加的作用，这是子痫前期的重要病理生理特征。
